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We are analyzing https://www.nature.com/articles/s41598-021-81557-1.

Title:
The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal | Scientific Reports
Description:
Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

cells, apoptotic, macrophages, pubmed, article, bacteria, bmdm, pak, efferocytic, aeruginosa, google, scholar, cas, efferocytosis, fig, cell, macrophage, control, central, min, bacterial, inflammatory, response, capacity, pseudomonas, resolution, targets, cytokine, inflammation, increase, stimuli, proinflammatory, index, phagocytic, infection, expression, data, nature, shown, immunol, presence, supplementary, image, performed, analysis, tissue, clearance, observed, material, results,

Topics {✒️}

nature portfolio privacy policy nature advertising genetic tools annexinv-alexa-647 single-labeled cells anti-il-6-neutralizing monoclonal antibody reprints research career members projected confocal z-stacks china bone marrow-derived macrophages lps-stimulated cd11b-positive bmdm pe anti-mouse cd11b anti-il-6-neutralizing monoclonal anti-lamp1 antibody 1d4b pro-resolving molecular pathways serum-free media apoptotic cell-based therapies major pro-inflammatory cytokine direct-zol rna miniprep strong pro-inflammatory profile zymo research research fellow real-time pcr system cfse-labeled apoptotic cells anti-viral immune responses40 differently-induced macrophage populations lamp1-positive vesicles original author pro-inflammatory cytokine expression anti-inflammatory cytokine il-10 permissions efferocytic receptor mertk anti-inflammatory/efferocytic phenotypes full size image confocal image stacks 3d-object counter plugin early pro-inflammatory response recombinant murine m-csf tagging gram-negative bacteria key anti-inflammatory mediator type-2-mediated tissue repair real-time rt-pcr microbicidal/pathogen killing phenotype stat3-il-10-il-6 pathway cytokine secretion profile adriana valeria jäger pseudomonas aeruginosa-infected macrophages research

Questions {❓}

  • Resolution of inflammation: What controls its onset?

Schema {🗺️}

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         headline:The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
         description:Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.
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      headline:The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
      description:Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.
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