DOI . ORG {
}
We began analyzing https://link.springer.com/article/10.1007/s11481-019-09874-x, but it redirected us to https://link.springer.com/article/10.1007/s11481-019-09874-x. The analysis below is for the second page.
Title[redir]:
Modern Techniques for the Isolation of Extracellular Vesicles and Viruses | Journal of Neuroimmune Pharmacology
Description:
Extracellular signaling is pivotal to maintain organismal homeostasis. A quickly emerging field of interest within extracellular signaling is the study of extracellular vesicles (EV), which act as messaging vehicles for nucleic acids, proteins, metabolites, lipids, etc. from donor cells to recipient cells. This transfer of biologically active material within a vesicular body is similar to the infection of a cell through a virus particle, which transfers genetic material from one cell to another to preserve an infection state, and viruses are known to modulate EV. Although considerable heterogeneity exists within EV and viruses, this review focuses on those that are small (< 200 nm in diameter) and of relatively low density (< 1.3 g/mL). A multitude of isolation methods for EV and virus particles exist. In this review, we present an update on methods for their isolation, purification, and phenotypic characterization. We hope that the information we provide will be of use to basic science and clinical investigators, as well as biotechnologists in this emerging field.
Title[redir]:
Modern Techniques for the Isolation of Extracellular Vesicles and Viruses | Journal of Neuroimmune Pharmacology
Description:
Extracellular signaling is pivotal to maintain organismal homeostasis. A quickly emerging field of interest within extracellular signaling is the study of extracellular vesicles (EV), which act as messaging vehicles for nucleic acids, proteins, metabolites, lipids, etc. from donor cells to recipient cells. This transfer of biologically active material within a vesicular body is similar to the infection of a cell through a virus particle, which transfers genetic material from one cell to another to preserve an infection state, and viruses are known to modulate EV. Although considerable heterogeneity exists within EV and viruses, this review focuses on those that are small (< 200 nm in diameter) and of relatively low density (< 1.3 g/mL). A multitude of isolation methods for EV and virus particles exist. In this review, we present an update on methods for their isolation, purification, and phenotypic characterization. We hope that the information we provide will be of use to basic science and clinical investigators, as well as biotechnologists in this emerging field.
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Keywords {🔍}
pubmed, article, google, scholar, cas, central, vesicles, extracellular, exosomes, virus, cells, sci, isolation, van, cell, human, hiv, virol, flow, extracell, methods, cancer, analysis, purification, httpsdoiorgs, nef, dittmer, proc, natl, acad, httpsdoiorgpnas, viruses, vesicle, viral, plasma, chromatography, rep, plos, exosome, content, particles, httpsdoiorgjevv, influenza, httpsdoiorgjvi, mcnamara, transfer, infection, rna, biol, meckes,
Topics {✒️}
rivera-serrano ee month download article/chapter epstein-barr virus lmp1 noncanonical nf-κb signaling cancer type-specific biomarkers tumor-growth promoting effect coated beads-based capture recombinant protein-based virus extracellular vesicle research multi-angle light scattering pseudo-stable transient transfection ready-made chromatography columns tumour-promoting stromal myofibroblasts clathrin-mediated endocytic pathway membrane affinity-based method quasi-enveloped human hepatoviruses van der pol dendritic cell-derived exosomes ultracentrifuge-based recovery procedures extracellular vesicle isolation protein trafficking full article pdf cross-flow based isolation extracellular vesicle heterogeneity endocytosis-competent extracellular vesicles b-cell exosome proteins nanoparticle-based flow virometry immune-modulatory features polyethylene glycol-based method rapid small-scale isolation sh-sy5y neural cells epstein-barr virus extracellular vesicle purification meckes dg jr tumor-derived micrornas high-purity extracellular vesicles trans-activating response oncolytic vaccinia virus size exclusion chromatography size-exclusion chromatography privacy choices/manage cookies article mcnamara hiv-1 life cycle magnetic anionic nanobeads extracellular inflammatory exosomes extracellular vesicle sampey gc augments stress response van der grein pro-inflammatory cytokines
Questions {❓}
- Webber J, Clayton A (2013) How pure are your vesicles?
Schema {🗺️}
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