DOI . ORG {}

Analyzed Page
Matching Content Categories
CMS
Monthly Traffic Estimate
How Does Doi.org Make Money
Keywords
Topics
Schema
External Links
Analytics And Tracking
Libraries
Hosting Providers

We began analyzing https://link.springer.com/article/10.1007/s11033-020-05892-8, but it redirected us to https://link.springer.com/article/10.1007/s11033-020-05892-8. The analysis below is for the second page.

Title[redir]:
Berberine reverses epithelial-mesenchymal transition and modulates histone methylation in osteosarcoma cells | Molecular Biology Reports
Description:
Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.

Matching Content Categories {📚}

Education
Science
Health & Fitness

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We find it hard to spot revenue streams.

Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Doi.org could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

article, pubmed, google, scholar, cas, osteosarcoma, cancer, berberine, cells, central, cell, transition, metastasis, epithelialmesenchymal, zhang, signaling, expression, epigenetic, data, varshney, emt, interest, human, chen, analysis, molecular, histone, roy, ecadherin, access, res, yang, roorkee, privacy, cookies, content, research, mishra, liu, oncol, number, mol, declares, conflict, information, publish, search, reverses, sircar, study,

Topics {✒️}

caspase-1/il-1β signaling axis β-catenin/lef-1 signaling pathway month download article/chapter pancreatic β-cell dysfunction epithelial-mesenchymal transition ampk/mtor signaling pathway inhibiting β-catenin signaling ampk/mtor-mediated lipophagy berberine reverses epithelial cancer stem cells epithelial–mesenchymal plasticity berberine inhibits proliferation osteosarcoma cell lines actin-binding proteins expression bone sarcoma cells modulates histone methylation tumor-induced angiogenesis related subjects mesenchymal transition full article pdf berberine induces apoptosis privacy choices/manage cookies berberine significantly reduced human osteosarcoma cells importantly reverses emt cancer cell invasion histone demethylase utx berberine pleiotropic effects berberine affects osteosarcoma writing—review & editing natural isoquinoline alkaloid frequently occurring cancer writing—original draft partha roy ritu varshney key epigenetic regulators colon cancer cells check access instant access colorectal cancer emt transcription factors inhibits metastasis european economic area recent years due diverse pharmacological effects wound healing ability end results program jones-wallace dj post-translational modifications endoplasmic reticulum stress

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Berberine reverses epithelial-mesenchymal transition and modulates histone methylation in osteosarcoma cells
         description:Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.
         datePublished:2020-10-19T00:00:00Z
         dateModified:2020-10-19T00:00:00Z
         pageStart:8499
         pageEnd:8511
         sameAs:https://doi.org/10.1007/s11033-020-05892-8
         keywords:
            Berberine
            Osteosarcoma
            Epithelial-mesenchymal transition
            Epigenetic
            Histone methylation
            Animal Biochemistry
            Animal Anatomy / Morphology / Histology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig5a_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig5b_HTML.png
         isPartOf:
            name:Molecular Biology Reports
            issn:
               1573-4978
               0301-4851
            volumeNumber:47
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Netherlands
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Rutusmita Mishra
               affiliation:
                     name:Indian Institute of Technology Roorkee
                     address:
                        name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Sandip Nathani
               affiliation:
                     name:Indian Institute of Technology Roorkee
                     address:
                        name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ritu Varshney
               url:http://orcid.org/0000-0002-2290-6263
               affiliation:
                     name:Indian Institute of Technology Roorkee
                     address:
                        name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Debabrata Sircar
               url:http://orcid.org/0000-0002-2788-119X
               affiliation:
                     name:Indian Institute of Technology Roorkee
                     address:
                        name:Plant Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Partha Roy
               url:http://orcid.org/0000-0003-1943-3079
               affiliation:
                     name:Indian Institute of Technology Roorkee
                     address:
                        name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Berberine reverses epithelial-mesenchymal transition and modulates histone methylation in osteosarcoma cells
      description:Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.
      datePublished:2020-10-19T00:00:00Z
      dateModified:2020-10-19T00:00:00Z
      pageStart:8499
      pageEnd:8511
      sameAs:https://doi.org/10.1007/s11033-020-05892-8
      keywords:
         Berberine
         Osteosarcoma
         Epithelial-mesenchymal transition
         Epigenetic
         Histone methylation
         Animal Biochemistry
         Animal Anatomy / Morphology / Histology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig5a_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs11033-020-05892-8/MediaObjects/11033_2020_5892_Fig5b_HTML.png
      isPartOf:
         name:Molecular Biology Reports
         issn:
            1573-4978
            0301-4851
         volumeNumber:47
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Netherlands
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Rutusmita Mishra
            affiliation:
                  name:Indian Institute of Technology Roorkee
                  address:
                     name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sandip Nathani
            affiliation:
                  name:Indian Institute of Technology Roorkee
                  address:
                     name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ritu Varshney
            url:http://orcid.org/0000-0002-2290-6263
            affiliation:
                  name:Indian Institute of Technology Roorkee
                  address:
                     name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Debabrata Sircar
            url:http://orcid.org/0000-0002-2788-119X
            affiliation:
                  name:Indian Institute of Technology Roorkee
                  address:
                     name:Plant Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Partha Roy
            url:http://orcid.org/0000-0003-1943-3079
            affiliation:
                  name:Indian Institute of Technology Roorkee
                  address:
                     name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Molecular Biology Reports
      issn:
         1573-4978
         0301-4851
      volumeNumber:47
Organization:
      name:Springer Netherlands
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Indian Institute of Technology Roorkee
      address:
         name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
         type:PostalAddress
      name:Indian Institute of Technology Roorkee
      address:
         name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
         type:PostalAddress
      name:Indian Institute of Technology Roorkee
      address:
         name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
         type:PostalAddress
      name:Indian Institute of Technology Roorkee
      address:
         name:Plant Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
         type:PostalAddress
      name:Indian Institute of Technology Roorkee
      address:
         name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Rutusmita Mishra
      affiliation:
            name:Indian Institute of Technology Roorkee
            address:
               name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
               type:PostalAddress
            type:Organization
      name:Sandip Nathani
      affiliation:
            name:Indian Institute of Technology Roorkee
            address:
               name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
               type:PostalAddress
            type:Organization
      name:Ritu Varshney
      url:http://orcid.org/0000-0002-2290-6263
      affiliation:
            name:Indian Institute of Technology Roorkee
            address:
               name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
               type:PostalAddress
            type:Organization
      name:Debabrata Sircar
      url:http://orcid.org/0000-0002-2788-119X
      affiliation:
            name:Indian Institute of Technology Roorkee
            address:
               name:Plant Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
               type:PostalAddress
            type:Organization
      name:Partha Roy
      url:http://orcid.org/0000-0003-1943-3079
      affiliation:
            name:Indian Institute of Technology Roorkee
            address:
               name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
      name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
      name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
      name:Plant Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
      name:Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(199)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

Emails and Hosting {✉️}

Mail Servers:

mx.zoho.eu
mx2.zoho.eu
mx3.zoho.eu

Name Servers:

josh.ns.cloudflare.com
zita.ns.cloudflare.com

5.12s.