We are analyzing https://link.springer.com/article/10.1007/s11033-020-05892-8.

Title:
Berberine reverses epithelial-mesenchymal transition and modulates histone methylation in osteosarcoma cells | Molecular Biology Reports
Description:
Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.
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Keywords {🔍}

article, pubmed, google, scholar, cas, osteosarcoma, cancer, berberine, cells, central, cell, transition, metastasis, epithelialmesenchymal, zhang, signaling, expression, epigenetic, data, varshney, emt, interest, human, chen, analysis, molecular, histone, roy, ecadherin, access, res, yang, roorkee, privacy, cookies, content, research, mishra, liu, oncol, number, mol, declares, conflict, information, publish, search, reverses, sircar, study,

Topics {✒️}

caspase-1/il-1β signaling axis β-catenin/lef-1 signaling pathway month download article/chapter pancreatic β-cell dysfunction epithelial-mesenchymal transition ampk/mtor signaling pathway inhibiting β-catenin signaling ampk/mtor-mediated lipophagy berberine reverses epithelial cancer stem cells epithelial–mesenchymal plasticity berberine inhibits proliferation osteosarcoma cell lines actin-binding proteins expression bone sarcoma cells modulates histone methylation tumor-induced angiogenesis related subjects mesenchymal transition full article pdf berberine induces apoptosis privacy choices/manage cookies berberine significantly reduced human osteosarcoma cells importantly reverses emt cancer cell invasion histone demethylase utx berberine pleiotropic effects berberine affects osteosarcoma writing—review & editing natural isoquinoline alkaloid frequently occurring cancer writing—original draft partha roy ritu varshney key epigenetic regulators colon cancer cells check access instant access colorectal cancer emt transcription factors inhibits metastasis european economic area recent years due diverse pharmacological effects wound healing ability end results program jones-wallace dj post-translational modifications endoplasmic reticulum stress

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         description:Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.
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      description:Osteosarcoma is the most frequently occurring cancer in children as well as young adolescents and the metastatic forms worsen this condition to a further great extent. The metastatic dissemination of cancer cells is often acquired through a process of epithelial-mesenchymal transition (EMT). Since, phytochemicals have attracted intense interest in recent years due to their diverse pharmacological effects, in the present study, we investigated if berberine, a naturally occurring isoquinoline quaternary alkaloid, could modulate the EMT in osteosarcoma cells. Our experimental studies showed that berberine reduced cell viability, colony formation, wound healing ability and migration of osteosarcoma cells. Also, berberine significantly reduced the expression of matrix metalloproteinase (MMP)-2, suggesting its inhibitory action on the matrix metalloproteinases that are required for cancer cell invasion. The significant reduction in the expression of vimentin, N-cadherin, fibronectin and increased expression of E-cadherin further suggested its role in the inhibition of EMT in osteosarcoma cells. The downregulation of H3K27me3, as well as the decreased expression of the histone methyl transferase enzyme EZH2, further substantiated the fact that the plant alkaloid can be used as an epigenetic modulator in the treatment of osteosarcoma. In conclusion, our findings suggest that berberine inhibits proliferation and migration of osteosarcoma cells and most importantly reverses EMT along with modulation of key epigenetic regulators.
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