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Title:
A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing | Nature Plants
Description:
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line overexpressing a strong growth-repressive gene, ETHYLENE RESPONSE FACTOR6 (ERF6). We identified six independent ethyl-methanesulfonate mutants rescuing the dwarfism of ERF6-overexpressing plants as a result of transgene silencing. Among the causative genes, ETHYLENE-INSENSITIVE5, SUPERKILLER2 and HASTY1 have previously been reported to inhibit PTGS. Notably, the three other causative genes have not, to date, been related to PTGS: UTP:RNA-URIDYLYLTRANSFERASE1 (URT1), C-TERMINAL DOMAIN PHOSPHATASE-LIKE3 (CPL3) and RESURRECTION1 (RST1). We show that these genes may participate in protecting the 3’ end of transgene transcripts. We present a model in which URT1, CPL3 and RST1 are classified as PTGS suppressors, as compromisation of these genes provokes the accumulation of aberrant transcripts which, in turn, trigger the production of small interfering RNAs, initiating RNA silencing. A study uses a forward genetic screen to identify three new regulators (URT1, CPL3 and RST1) for post-transcriptional gene silencing (PTGS). These regulators may suppress PTGS by protecting the 3’ end of transgene transcripts.
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nature portfolio journals nature portfolio permissions reprints privacy policy flanders research foundation c-terminal domain phosphatase-like3 advertising research stimulating scientific environment c-terminal domain phosphatase nature plants social media catalytic c-terminal domain protein fiery2/cpl1 interacts nature+ nature 431 nature transcriptional silencing induced strong growth-repressive gene cuticular wax biosynthesis srna sequencing data rna-mediated gene silencing rna-binding proteins hyl1 mapk phosphorylation-induced stabilization chimeric gene-transposon transcripts mrna sequencing data sirna‐mediated chromatin silencing context-dependent dual role plant systems biology rna quality control author correspondence references baulcombe sirna-mediated ptgs defense université de bordeaux nonsense-mediated mrna decay forward genetics screen nuclear quality control institutional subscriptions read water-limiting conditions springerlink instant access cytoplasmic sgs3/rdr6‐bodies silenced post‐transcriptionally post-doctoral fellow author fiery2/ctd phosphatase model data analysis stress response pathway systems biology forwards genetics screen cytosolic rna exosome
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