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Title[redir]:
Arabidopsis DCP2, DCP1, and VARICOSE Form a Decapping Complex Required for Postembryonic Development - PMC
Description:
mRNA turnover in eukaryotes involves the removal of m7GDP from the 5โฒ end. This decapping reaction is mediated by a protein complex well characterized in yeast and human but not in plants. The function of the decapping complex in the development of ...
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Keywords {๐}
dcp, decapping, vcs, arabidopsis, mutants, mrna, doi, pubmed, google, scholar, transcripts, figure, proteins, complex, rna, protein, pmc, cell, article, activity, parker, yeast, bodies, cells, free, mutant, development, vivo, phenotype, analysis, vitro, foci, mbpdcp, seedlings, plant, human, plants, domain, found, transcript, form, required, mrnas, suggesting, cytoplasmic, accumulation, shown, wild, type, decappingdeficient,
Topics {โ๏ธ}
typical lob-neck structure small homeodomain-leucine zipper pmc beta search xve-yfp-dcp1 coexpression lines t-dna insertion lines putative coiled-coil motif kh rna-binding domains t-dna left border differentiation-promoting transcription factor rna-dependent rna polymerase t-dna insertion sites full-length cfp-vcs decapping complex/machinery consists nonsense-free transcripts nonsense-mediated mrna decay mirna-mediated gene silencing dcp1โcyan fluorescent protein promoter-gus fusion constructs ptc-free forms supplementary materials included dcp1โred fluorescent protein 35s-yfp-vcsc line anti-gst monoclonal antibody hd-zipiii family mrnas nonsense-mediated decay pathway org/cgi/doi/10 full-length proteins vcs n-terminal region 35s-eif4e-cfp line sp dcp1-deficient mutants cap-labeled rnas generated dcp2/dcp1/vcs complex based beliakova-bethell 35s-yfp-vcsc cells scanning electron microscopy hedls/ge-1 protein structure decapping-deficient mutants compared vcs c-terminal region includes wild-type seedlings decapping complexโdeficient mutants mutant mbp-dcp2 bearing exact insertion site detect wild-type dcp1 decapping-deficient mutants suggests steady state nonsense mbp-dcp2 fusion protein p-body component gw182 agrobacterium-mediated transformation publisher site pdf license information pmcid
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