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Title[redir]:
AGO1 Homeostasis Entails Coexpression of MIR168 and AGO1 and Preferential Stabilization of miR168 by AGO1 - PMC
Description:
Arabidopsis ARGONAUTE1 (AGO1) encodes the RNA slicer enzyme of the microRNA (miRNA) pathway and is regulated by miR168-programmed, AGO1-catalyzed mRNA cleavage. Here, we describe two additional regulatory processes required for AGO1 homeostasis: ...
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Keywords {🔍}
ago, doi, plants, levels, rna, mago, mirna, figure, mira, accumulation, pubmed, google, scholar, mrna, wildtype, mirnas, sxmira, transformants, loop, col, pmc, arabidopsis, plant, promoter, bartel, article, increased, control, expression, dcl, complementary, free, vaucheret, analysis, mutants, cell, genes, blot, loading, construct, stemloop, mallory, pathway, proper, processed, results, sensitive, microrna, cleavage, level,
Topics {✒️}
org/cgi/content/full/22/1/129/dc1/ inverted t-dna repeat genome-wide insertional mutagenesis supplementary materials included trans-acting sirna biogenesis mir168-guided ago1-catalyzed cleavage pmc beta search p1/hc-pro full-length ago1 cdna fine-tuned posttranscriptional adjustment micro-rna-directed phasing class iii hd-zip stem-loop intermediate correlates generate mir168a-overexpressing plants p35s-mir168a-t35s fragment post-transcriptional gene silencing 4m-mir168a constructs deriving nihms37470-supplement-supplement independent wild-type plants planta agrobacterium-mediated transformation 0m-ago1 genomic construct altered stem-loop intermediate mir168a stem-loop intermediate mutagenized pac1-kpn1 fragment free ago1 offsets mir168a-loop probes reveals representative 35sx2-mir168 transformants ago1-catalyzed mrna cleavage 4m-ago1 plants exhibiting 4m-ago1 plants prevents wild-type ago1 gene increased free ago1 arabidopsis micro-rna biogenesis stem-loop intermediate accumulation wild-type col plants supplemental data wild-type ago1 mrna ectopic dicer-like1 expression wild-type col plant posttranscriptional ago1-mediated stabilization 0m-ago1 plants compared 0m-ago1 rna observed exhibited leaf serration wild-type col leaves severe 4m-ago1 phenotype t-dna copy primary mir168a transcript data availability statements hypomorphic ago1 alleles representative transformants carrying
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