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Keywords {🔍}

uridylation, doi, mrna, mrnas, figure, decay, rna, polya, pubmed, google, scholar, pmc, article, short, cells, free, tail, length, cell, atails, uridylated, factors, shown, knockdown, tailseq, degradation, chang, rnas, biol, end, histone, complex, mol, frequency, decapping, reads, targets, dcp, tails, control, atail, test, protein, data, utr, deadenylated, human, norbury, deadenylation, halflife,

Topics {✒️}

figure 1 nihms751375-supplement-table_s1__tail-seq_data_from_tut4_7-depleted_cells__related_to_figure_1_ figure 4 nihms751375-supplement-table_s2__measurement_of_mrna_half-life_following_tut4_7_knockdown_by_mrna-seq__related_to_figure_4_ supplemental information nihms751375-supplement-document_s1__article_plus_supplemental_information_ study nihms751375-supplement-table_s3__lists_of_oligonucleotides_used_in_this_study_ oligo-u-tail triggers decay pmc beta search a-tail length-dependent manner template-independent ribonucleotidyl transferases transcriptome-wide analyses allowed tail-seq protocol begins flag-tutase expression plasmids mirna-mediated gene silencing bear short u-tails method called tail-seq mirna-directed cleavage products gene-level analyses revealed references ameres sl full-length pabp occupies oligo-dt enrichment step nucleic acid-binding domain tailed paired mann-whitney carry terminal u-tails supplementary materials included small-scale tail-seq mirna-induced gene silencing rna-specific ribonucleotidyl transferases uridylation-independent alternative pathways tail-seq experiment shows nat rev genet genomic a-rich sequences immunopurified full-length tutases generate oligo-u-tails uridylation-dependent mrna decay experimental procedures construction uridylate single-stranded rnas full-length tut7 protein overexpressed dominant-negative mutants anti-pabpc1 antibody extended experimental procedures oligo-a-tail serves short a-tailed mrnas short a-tail length short a-tail range nucleotidyl-transferase superfamily c-terminal half nucleotidyl transferase domain average mrna half-life mrna half-life observed degrade mrna exonucleolytically measured mrna half-life

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