We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-971-0_4.

Title:
Detection of Uridylated mRNAs | SpringerLink
Description:
Uridine addition at the 3′ end of RNAs (i.e., uridylation) emerges as a critical posttranscriptional modification promoting RNA degradation. Uridylation has been notably linked to the degradation of small RNAs, correlated with the 5′ shortening of...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Detection of Uridylated mRNAs
      pageEnd:51
      pageStart:43
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-971-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Polyadenylation
         isbn:
            978-1-62703-971-0
            978-1-62703-970-3
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:François M. Sement
            affiliation:
                  name:Université de Strasbourg
                  address:
                     name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Dominique Gagliardi
            affiliation:
                  name:Université de Strasbourg
                  address:
                     name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Uridylation, 3′RACE PCR, mRNA, Noncanonical poly(A) polymerase, RNA degradation
      description:Uridine addition at the 3′ end of RNAs (i.e., uridylation) emerges as a critical posttranscriptional modification promoting RNA degradation. Uridylation has been notably linked to the degradation of small RNAs, correlated with the 5′ shortening of RISC-cleaved transcripts and the degradation of mRNAs. We describe here a method based on 3′ RACE (3′ Rapid Amplification of cDNA End) PCR that has been successfully used to investigate nucleotide addition at the 3′ end of RISC-cleaved transcripts and full-length mRNAs in plants.
      datePublished:2014
      isAccessibleForFree:
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Book:
      name:Polyadenylation
      isbn:
         978-1-62703-971-0
         978-1-62703-970-3
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Université de Strasbourg
      address:
         name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
         type:PostalAddress
      name:Université de Strasbourg
      address:
         name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:François M. Sement
      affiliation:
            name:Université de Strasbourg
            address:
               name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
               type:PostalAddress
            type:Organization
      name:Dominique Gagliardi
      affiliation:
            name:Université de Strasbourg
            address:
               name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
      name:Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg Cedex, France
WebPageElement:
      isAccessibleForFree:
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