We are analyzing https://link.springer.com/article/10.1186/bcr1982.

Title:
Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy | Breast Cancer Research
Description:
Introduction The phenotypic and functional differences between cells that initiate human breast tumors (cancer stem cells) and those that comprise the tumor bulk are difficult to study using only primary tumor tissue. We embarked on this study hypothesizing that breast cancer cell lines would contain analogous hierarchical differentiation programs to those found in primary breast tumors. Methods Eight human breast cell lines (human mammary epithelial cells, and MCF10A, MCF7, SUM149, SUM159, SUM1315 and MDA.MB.231 cells) were analyzed using flow cytometry for CD44, CD24, and epithelial-specific antigen (ESA) expression. Limiting dilution orthotopic injections were used to evaluate tumor initiation, while serial colony-forming unit, reconstitution and tumorsphere assays were performed to assess self-renewal and differentiation. Pulse-chase bromodeoxyuridine (5-bromo-2-deoxyuridine [BrdU]) labeling was used to examine cell cycle and label-retention of cancer stem cells. Cells were treated with paclitaxol and 5-fluorouracil to test selective resistance to chemotherapy, and gene expression profile after chemotherapy were examined. Results The percentage of CD44+/CD24- cells within cell lines does not correlate with tumorigenicity, but as few as 100 cells can form tumors when sorted for CD44+/CD24-/low/ESA+. Furthermore, CD44+/CD24-/ESA+ cells can self-renew, reconstitute the parental cell line, retain BrdU label, and preferentially survive chemotherapy. Conclusion These data validate the use of cancer cell lines as models for the development and testing of novel therapeutics aimed at eradicating cancer stem cells.
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Topics {✒️}

cd44+/cd24-/esa+breast cancer stem cd44+/cd24-/low/epithelial-specific antigen cd44+/cd24-/low/esa+ sorted cells cd44+/cd24-/low/esa+ subpopulation cd44+/cd24-/esa+ stem cells cd44+/cd24-/esa+ cells ranges generate cd44+/cd24+/esa+ cells viable cd44+/cd24-/esa+ cells target cd44+/cd24-/esa+ cells cd44+/cd24-/low/esa+ cells cd44+cd24-esa+ phenotype enriches cd44+/cd24-/low/esa+ subpopulations cd44+/cd24-/low/esa+ phenotype [2] cd44+/cd24-/low/esa+ fraction cd44+/cd24-/esa+cells cycle cd44+/cd24-/esa- sorted cells cd44+/cd24+/esa+ sorted cells sorted cd44+/cd24-/esa+ cells cd44+/cd24+/esa+ cells exhibited cd44+/cd24-/esa- colonies exhibited cd44+/cd24-/esa+ cells detected cd44+/cd24-/esa- populations formed cd44+/cd24-/low phenotype enrich stem/progenitor cell properties cd44+/cd24-/esa- basal cells characterize stem/progenitor cells cd44+/cd24-/low/esa+ 000 cd44+/cd24-/low/esa+ 000 cd44+/cd24-/low/esa cd44+cd24-esa+ subpopulation super-low adherence plates article download pdf cd44+/cd24-/low cells breast cancer-initiating cells fluorescence-activated cell sorting cd44+/cd24-/esa- fractions cd44+/cd24+/esa+ fractions high-resolution genomic profiles cd44+/cd24-cells associates stem-cell biology stem cell biology label-retaining epithelial cells cd44+/cd24-/esa+ cells cd44+/cd24-/esa- cells cd44+/cd24+/esa+ cells cd44+/cd24-//esa- cells cd44+/cd24-/esa+cells 30% cd44+/cd24-/esa+ cells cd44+/cd24-/esa+ sorted cancer-derived cell lines

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