
NCBI . NLM . NIH . GOV {
}
Title[redir]:
In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells - PMC
Description:
Although the existence of mammary stem cells has been suggested by serial transplantation studies in mice, their identification has been hindered by the lack of specific surface markers, and by the absence of suitable in vitro assays for testing ...
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Keywords {๐}
cells, mammospheres, stem, doi, cell, mammary, google, scholar, pubmed, epithelial, differentiation, expression, human, genes, fig, conditions, growth, single, markers, culture, suspension, differentiated, development, generated, cancer, breast, colonies, gland, population, mammosphere, collagen, progenitor, expressed, ability, structures, normal, grown, secondary, spheres, matrigel, tissue, cytokeratin, table, pmc, vitro, cultivation, undifferentiated, analysis, potential, number,
Topics {โ๏ธ}
transplanted immune-deficient nod/scid real-time rtโpcr reaction real-time rtโpcr data real-time rtโpcr real time rt-pcr serine/threonine kinase 17a include active jak/stat fire-polished pasteur pipette 1ร pen/strep/fungizone mix stem/progenitor cell-specific functions pmc beta search cd7+cd34โ lymphoid progenitor texas-red-labeled secondary antibodies small inducible-cytokine a2 quantitative rtโpcr showed dome-shaped structures formed retroviral vectors plegfp-c1 real-time pcr free-floating cells tissue-culture-treated plastic mammary stem/progenitor cells double-strand cdna synthesis stem/progenitor cell functionality representative positive-staining sphere generating ductal-acinar structures early progenitor/stem cells serum-free medium cell-surface markers specific serum-free conditions abc transporter bcrp1/abcg2 secondary mammosphere-derived cell branched-acinar structures formed identified mouseโhuman pairs stem/progenitor cell markers entire ductal-acinar architecture nih grant ca075136 long-term growth rneasy mini kit mammosphere-derived cells transferred eventually undergo senescence nod/scid/b2m ductal-acinar structure generated human breast cancers mammosphere-derived cells compared mammosphere-derived cells grown publisher site pdf yield single cells assessed lineage-specific differentiation overlapping genetic programs license information pmcid
Questions {โ}
- Is there a neural stem cell in the mammalian forebrain?
- The evolving concept of a stem cell: Entity or function?
- Wnt signaling in breast cancer: Have we come full circle?
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