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Title[redir]:
Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome | Nature Chemical Biology
Description:
A method called m1A-ID-seq, which involves antibody enrichment and reverse transcriptase–based sequencing of N1-methyladenosine (m1A) RNA modifications, reveals that m1A is a reversible mRNA modification that is abundant in the 5′ UTRs of human genes. N1-Methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, yet little is known about its abundance, topology and dynamics in mRNA. Here, we show that m1A is prevalent in Homo sapiens mRNA, which shows an m1A/A ratio of ∼0.02%. We develop the m1A-ID-seq technique, based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, as a means for transcriptome-wide m1A profiling. m1A-ID-seq identifies 901 m1A peaks (from 600 genes) in mRNA and noncoding RNA and reveals a prominent feature, enrichment in the 5′ untranslated region of mRNA transcripts, that is distinct from the pattern for N6-methyladenosine, the most abundant internal mammalian mRNA modification. Moreover, m1A in mRNA is reversible by ALKBH3, a known DNA/RNA demethylase. Lastly, we show that m1A methylation responds dynamically to stimuli, and we identify hundreds of stress-induced m1A sites. Collectively, our approaches allow comprehensive analysis of m1A modification and provide tools for functional studies of potential epigenetic regulation via the reversible and dynamic m1A methylation.
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Keywords {🔍}
pubmed, article, google, scholar, cas, rna, nature, central, nmethyladenosine, mrna, methylation, modification, cell, wang, access, trna, nat, content, chemical, reveals, nucleic, res, human, chem, dynamic, acids, biol, china, cookies, analysis, xiong, methods, dna, supplementary, privacy, data, biology, reversible, xushen, chengqi, mammalian, methyltransferase, modified, authors, research, information, xiaoyu, xiaoting, shu, shiqing,
Topics {✒️}
nature portfolio plant gene research permissions reprints privacy policy advertising n-1-methyl-adenosine base modification provide tools social media dynamic n1-methyladenosine methylome n1-methyl-adenosine modification n6-methyladenosine-dependent regulation nature http spring-loaded base modification single-nucleotide-resolution mapping rna n6-methyladenosine methyltransferase nature 278 nature 419 nature 421 nature 485 nature 526 nature 518 nature 505 nature wild-type hek293t cells m1a-id-seq technique transcriptome-wide m1a profiling dna alkylation damage serum starvation-induced m1a high-resolution genomic analysis author correspondence n1-methyladenosine modification posttranscriptional rna modifications springerlink instant access dynamic m1a methylation permissions genome-wide analysis m6a writers reveals personal data stress-induced m1a sites site-specific methylation ribosomal rna methylation article li mammalian rna demethylase eukaryotic messenger rna messenger rna stability dna/rna demethylase chemical biology data protection reverse transcription-based methods recombinant alkbh3 protein
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headline:Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome
description:A method called m1A-ID-seq, which involves antibody enrichment and reverse transcriptaseâbased sequencing of N1-methyladenosine (m1A) RNA modifications, reveals that m1A is a reversible mRNA modification that is abundant in the 5â² UTRs of human genes.
N1-Methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, yet little is known about its abundance, topology and dynamics in mRNA. Here, we show that m1A is prevalent in Homo sapiens mRNA, which shows an m1A/A ratio of â¼0.02%. We develop the m1A-ID-seq technique, based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, as a means for transcriptome-wide m1A profiling. m1A-ID-seq identifies 901 m1A peaks (from 600 genes) in mRNA and noncoding RNA and reveals a prominent feature, enrichment in the 5â² untranslated region of mRNA transcripts, that is distinct from the pattern for N6-methyladenosine, the most abundant internal mammalian mRNA modification. Moreover, m1A in mRNA is reversible by ALKBH3, a known DNA/RNA demethylase. Lastly, we show that m1A methylation responds dynamically to stimuli, and we identify hundreds of stress-induced m1A sites. Collectively, our approaches allow comprehensive analysis of m1A modification and provide tools for functional studies of potential epigenetic regulation via the reversible and dynamic m1A methylation.
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headline:Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome
description:A method called m1A-ID-seq, which involves antibody enrichment and reverse transcriptaseâbased sequencing of N1-methyladenosine (m1A) RNA modifications, reveals that m1A is a reversible mRNA modification that is abundant in the 5â² UTRs of human genes.
N1-Methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, yet little is known about its abundance, topology and dynamics in mRNA. Here, we show that m1A is prevalent in Homo sapiens mRNA, which shows an m1A/A ratio of â¼0.02%. We develop the m1A-ID-seq technique, based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, as a means for transcriptome-wide m1A profiling. m1A-ID-seq identifies 901 m1A peaks (from 600 genes) in mRNA and noncoding RNA and reveals a prominent feature, enrichment in the 5â² untranslated region of mRNA transcripts, that is distinct from the pattern for N6-methyladenosine, the most abundant internal mammalian mRNA modification. Moreover, m1A in mRNA is reversible by ALKBH3, a known DNA/RNA demethylase. Lastly, we show that m1A methylation responds dynamically to stimuli, and we identify hundreds of stress-induced m1A sites. Collectively, our approaches allow comprehensive analysis of m1A modification and provide tools for functional studies of potential epigenetic regulation via the reversible and dynamic m1A methylation.
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