
NCBI . NLM . NIH . GOV {
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Title[redir]:
N6-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO - PMC
Description:
We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N6-methyladenosine (m6A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m6A in mRNA, ...
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Keywords {🔍}
fto, doi, supplementary, fig, mrna, google, scholar, pubmed, pmc, rna, cells, article, nuclear, demethylation, activity, free, speckles, observed, biol, level, cell, nat, substrate, ssrna, cellular, actd, nlm, abh, analysis, splicing, pol, iisp, transcription, foci, chem, oxidative, knockdown, shown, hela, total, inhibition, science, ncbi, search, protein, decreased, physiological, function, ssdna, data,
Topics {✒️}
genome-wide association studies α-kg-dependent manner alkb family proteins cbc/uic rrc proteomics pmc beta search site-specifically incorporated m6a fto-activity dependent manner wild-type fto showed single-stranded nucleic acids15 p-body marker dcp1a alkb family proteins14 lc-ms/ms analysis pol ii-s2p transcription wild-type fto decreased overexpressed wild-type fto fto-mediated oxidative demethylation nih eureka award oxidatively demethylate m3t post-transcriptional splicing dominating methylated base [google scholar] 2 wild-type activity reversible chemical modification active site mutations replication site pcna α-kg ligands lc-ms/ms mrna n6-adenosine-methyltransferase nat chem biol pol ii-s2p pol–ii s2p telomere marker trf1 recent crystal structure requires detailed investigation completely demethylate m6a fto sirna-treated hela pol ii-s2p21 fto partially colocalizes rna stem loop quantitative foci analysis fto remains unknown united states government license information pmcid single-stranded dna exceedingly low compared standard curve generated u5 tri-snrnp discrete speckle foci white arrows correspond chicago biomedical consortium
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