
NCBI . NLM . NIH . GOV {
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Title[redir]:
ARM-Seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments - PMC
Description:
High throughput RNA sequencing has accelerated discovery of the complex regulatory roles of small RNAs, but RNAs containing modified nucleosides may escape detection when those modifications interfere with reverse transcription during RNA-seq ...
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Keywords {π}
doi, trnas, armseq, rna, trna, rnas, pubmed, google, scholar, pmc, article, modifications, human, free, mature, supplementary, modification, sequencing, small, reads, primer, read, sequences, modified, yeast, figure, trnaderived, samples, alkb, mitochondrial, cell, fragments, extension, evidence, modomics, data, gene, derived, untreated, genes, table, mapping, sequence, nucleic, acids, res, detection, state, including, profiles,
Topics {βοΈ}
human leu-caa pre-trna post-transcriptional processing steps significant arm-seq responses post-transcriptional rna modifications trna-derived small rnas5 progressive motor-neuron loss small trna-derived rnas trna-derived small rnas produce full-length cdnas examined arm-seq results arm-seq correctly predicted pmc beta search arm-seq greatly increases trna-ala-agc-1-3 describe fast gapped-read alignment arm-seq facilitates sequencing fragmentary pre-trnas revealed called βhard-stopβ modifications frequent hard-stop modifications trna-derived rna fragments full-length trnas comprised leu-taa-1 & lys-ctt-1 arm-seq read profiles supplementary figures s7βs11 arm-seq abundance ratios arm-seq results reflect trna anti-codon loop reverse transcription-based methods full-length mature trnas expected hard-stop modifications post-transcriptional modifications reporting arm-seq fold rna-seq read counts rna-seq library preparation rna-seq reads mapping rna-seq library inclusion primer extensions targeting showed significant responses small rnas derived prevalent hard-stop modification arm-seq demethylation increased high-throughput sequencing initiator methionine pre-trnas arm-seq responses multiple gene loci revealing tissue-specific functions high-throughput annotation efficiently revealed modifications escape sequencing-based detection methyl-modified rnas
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