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We are analyzing https://www.nature.com/articles/nchembio.2040.

Title:
Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome | Nature Chemical Biology
Description:
A method called m1A-ID-seq, which involves antibody enrichment and reverse transcriptase–based sequencing of N1-methyladenosine (m1A) RNA modifications, reveals that m1A is a reversible mRNA modification that is abundant in the 5′ UTRs of human genes. N1-Methyladenosine (m1A) is a prevalent post-transcriptional RNA modification, yet little is known about its abundance, topology and dynamics in mRNA. Here, we show that m1A is prevalent in Homo sapiens mRNA, which shows an m1A/A ratio of ∼0.02%. We develop the m1A-ID-seq technique, based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, as a means for transcriptome-wide m1A profiling. m1A-ID-seq identifies 901 m1A peaks (from 600 genes) in mRNA and noncoding RNA and reveals a prominent feature, enrichment in the 5′ untranslated region of mRNA transcripts, that is distinct from the pattern for N6-methyladenosine, the most abundant internal mammalian mRNA modification. Moreover, m1A in mRNA is reversible by ALKBH3, a known DNA/RNA demethylase. Lastly, we show that m1A methylation responds dynamically to stimuli, and we identify hundreds of stress-induced m1A sites. Collectively, our approaches allow comprehensive analysis of m1A modification and provide tools for functional studies of potential epigenetic regulation via the reversible and dynamic m1A methylation.
Website Age:
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Keywords {🔍}

pubmed, article, google, scholar, cas, rna, nature, central, nmethyladenosine, mrna, methylation, modification, cell, wang, access, trna, nat, content, chemical, reveals, nucleic, res, human, chem, dynamic, acids, biol, china, cookies, analysis, xiong, methods, dna, supplementary, privacy, data, biology, reversible, xushen, chengqi, mammalian, methyltransferase, modified, authors, research, information, xiaoyu, xiaoting, shu, shiqing,

Topics {✒️}

nature portfolio plant gene research permissions reprints privacy policy advertising n-1-methyl-adenosine base modification provide tools social media dynamic n1-methyladenosine methylome n1-methyl-adenosine modification n6-methyladenosine-dependent regulation nature http spring-loaded base modification single-nucleotide-resolution mapping rna n6-methyladenosine methyltransferase nature 278 nature 419 nature 421 nature 485 nature 526 nature 518 nature 505 nature wild-type hek293t cells m1a-id-seq technique transcriptome-wide m1a profiling dna alkylation damage serum starvation-induced m1a high-resolution genomic analysis author correspondence n1-methyladenosine modification posttranscriptional rna modifications springerlink instant access dynamic m1a methylation permissions genome-wide analysis m6a writers reveals personal data stress-induced m1a sites site-specific methylation ribosomal rna methylation article li mammalian rna demethylase eukaryotic messenger rna messenger rna stability dna/rna demethylase chemical biology data protection reverse transcription-based methods recombinant alkbh3 protein

Schema {🗺️}

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            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Xushen Xiong
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Academy for Advanced Interdisciplinary Studies, Peking University
            address:
               name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Kun Wang
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Lixia Wang
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Xiaoting Shu
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Academy for Advanced Interdisciplinary Studies, Peking University
            address:
               name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Shiqing Ma
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Academy for Advanced Interdisciplinary Studies, Peking University
            address:
               name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      name:Chengqi Yi
      affiliation:
            name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University
            address:
               name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Peking-Tsinghua Center for Life Sciences, Peking University
            address:
               name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
            name:Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University
            address:
               name:Department of Chemical Biology, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China
      name:State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
      name:Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
      name:Department of Chemical Biology, Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, China
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