NCBI . NLM . NIH . GOV {
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Title[redir]:
Efficient and quantitative high-throughput transfer RNA sequencing - PMC
Description:
Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced by standard methods due to abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieve efficient and ...
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Keywords {๐}
trna, doi, rna, pmc, sequencing, pubmed, google, scholar, total, demethylase, fig, article, treatment, reads, trnas, modification, supplementary, free, methods, expression, alkb, cdna, cells, position, genes, modifications, structure, group, purified, nlm, quantitative, acids, reaction, demethylation, stops, performed, synthesis, positions, tgirt, templateswitching, number, nature, pmol, data, ncbi, search, library, nat, standard, remove,
Topics {โ๏ธ}
initial template-primer substrate read2 primer-binding sites substrate-bound alkb17 revealed ni-nta superflow cartridge d-tube dialyzer maxi high-throughput rna sequencing phenol-chloroform-isoamyl alcohol tgirt template-switching reactions adds rna-seq adaptors numerous post-transcriptional modifications pmc beta search rna-seq methods start quantitative trna-seq feasible catalytically active alkb-ฮดn11 qqq lc-ms wild-type enzyme alkb quantitative trna-seq methods single-stranded nucleic acids abundant post-transcriptional modifications high throughput manner full-length trna reads blue line shows dm-tgirt-seq method performed ph-activity profiling nat methods abstract highly structured trna rna repair function5 barcode gel-purified total trna license information pmcid phenolโchloroform extraction wild-type protein quantitative trna sequencing mammalian cell culture substantial rt stop array results showed alleviate potential interference nih grants r01gm37949 full-length trna wild-type alkb gene copy number rt stops due standard sequencing methods supplementary materials included full-length cdnas standard rna-seq genomic trna database full-length reads passive gel elution standard methods due
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