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Title:
Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells | Nature Communications
Description:
Krüppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in cell cycle regulation, somatic cell reprogramming and pluripotency. Here we focus on the functional divergence between Klf4 and Klf5 in the inhibition of mouse embryonic stem (ES) cell differentiation. Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, we show that Klf4 negatively regulates the expression of endodermal markers in the undifferentiated ES cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. Knockdown of Klf4 enhances differentiation towards visceral and definitive endoderm. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which control commitment to the mesoderm lineage, and knockdown of Klf5 specifically enhances differentiation towards mesoderm. We conclude that Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in murine ES cells. The Krüppel-like factors Klf4 and Klf5 have wide roles in cell biology including the regulation of pluripotency. In this study, Aksoy et al. demonstrate that Klf4 preferentially blocks the expression of endodermal markers in embryonic stem cells, whereas Klf5 negatively regulates mesodermal factors.
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klf, cells, differentiation, expression, klfkd, endoderm, article, control, cell, fig, google, scholar, genes, mesoderm, markers, knockdown, cas, sox, gata, stem, esgscgfpsoxhucdklfkd, mouse, definitive, klfoe, clones, mixl, embryonic, factors, endodermal, visceral, mesodermal, gene, performed, supplementary, increased, analysis, results, observed, teratomas, nature, gfp, fold, expressing, level, data, induced, day, showed, promoter, nanog,
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nature portfolio permissions reprints privacy policy fluorescein-anti-mouse e-cadherin mab es-gscgfpsox17hucd25-klf5-kd clones exhibited advertising es-gscgfpsox17hucd25-klf4-kd clones exhibited ultra-high-throughput dna sequencing es-gscgfpsox17hucd25-klf4-kd cells exhibited es-gscgfpsox17hucd25-klf5-kd clones showed es-gscgfpsox17hucd25-klf4-kd clones showed south china institute apc-anti-human cd25 mab lif-deprived serum-free medium brain research institute apc-anti-mouse flk1 mab social media rnaeasy kits sox17-dsred-klf5-kd es cells pierre-yves bourillot michael xavier doss resulting es-gscgfpsox17hucd25-klf4-kd nature 343 nature 489 nature es-gscgfpsox17hucd25-klf5-kd clones es-gscgfpsox17hucd25-klf4-kd cells apc-anti-mouse cd140a evolutionarily conserved zinc-finger full access produce siv-derived lentivectors klf5-kd cells-derived teratomas context-dependent opposing forces klf4-kd cells-derived teratomas collagen iv-coated dishes klf5-kd2 cells-derived teratomas accession numbers e-mtab-1838 pgae-cag-egfp-wpre46 research grants generated es-gscgfpsox17hucd25 subclones quantitative real-time pcr embryo-derived stem cells extracellular cysteine-rich domain es-gscgfpsox17hucd25-klf5-kd real-time pcr analysis identify mucin-expressing epithelium klf5-kd clones exhibited es-gscgfpsox17hucd25-klf4-kd pre-implantation mouse embryo pgae-cag-klf5-wpre
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headline:Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells
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Cell signalling
Embryogenesis
Stem-cell differentiation
Science
Humanities and Social Sciences
multidisciplinary
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headline:Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells
description:Krüppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in cell cycle regulation, somatic cell reprogramming and pluripotency. Here we focus on the functional divergence between Klf4 and Klf5 in the inhibition of mouse embryonic stem (ES) cell differentiation. Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, we show that Klf4 negatively regulates the expression of endodermal markers in the undifferentiated ES cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. Knockdown of Klf4 enhances differentiation towards visceral and definitive endoderm. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which control commitment to the mesoderm lineage, and knockdown of Klf5 specifically enhances differentiation towards mesoderm. We conclude that Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in murine ES cells. The Krüppel-like factors Klf4 and Klf5 have wide roles in cell biology including the regulation of pluripotency. In this study, Aksoy et al. demonstrate that Klf4 preferentially blocks the expression of endodermal markers in embryonic stem cells, whereas Klf5 negatively regulates mesodermal factors.
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Science
Humanities and Social Sciences
multidisciplinary
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