
DOI . ORG {
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Title[redir]:
Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening | BMC Plant Biology | Full Text
Description:
Background The oil palm (Elaeis guineensis) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Results Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2β-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. Conclusions This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.
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Keywords {π}
trna, oil, palm, modifications, article, pubmed, google, scholar, analysis, rna, fruit, lipid, fig, phase, cas, modification, nucleosides, genes, development, correlation, trnas, central, total, mass, min, content, nucleoside, represents, data, lipids, plant, enzymes, full, china, acid, phases, material, bmc, fruits, specific, mesocarp, genome, enzyme, table, tgs, dynamic, elaeis, guineensis, identified, size,
Topics {βοΈ}
hplc-coupled mass spectrometry electronic supplementary material trna-mediated codon-biased translation high-resolution mass spectrometry n4-acetyl-2β-o-methylcytidine s2c 2-methylthio-n6-cis-hydroxyisopentenyladenosine m1a high-resolution mass spectrometer d-h2o fragments 2-methylthio-cis-zeatin riboside methyl tert-butyl ether gel sections chimeric autophagy-tethering compounds state privacy rights urea-page analysis additional information publisher full size image maldi mass spectrometry hplc-grade organic reagents nucleus-encoded transfer rnas bmc plant biol n6-cis-hydroxyisopentenyladenosine ms2i6a user-friendly online tool 5-carboxymethylaminomethyl-2β-o-methyluridine ncm5u 2-methylthio-n6-methyladenosine t6a trna-modifying enzyme genes productive oil-bearing crop post-transcriptional rna modifications 2-methylthio-n6-threonylcarbamoyladenosine ar supplementary material 10% urea-page sharing co2-sensitive trna modification 2-methylthio-n6-isopentenyladenosine ms2io6a chromosome-level reference genome urea-polyacrylamide gel electrophoresis widely accepted notion mass spectrometry privacy choices/manage cookies rice ostrm13 adds methods materials mass accuracy finer springer nature discussions identification identifying unique fragments comprehensive mapping reveals modified rna fragments exact mass precisely modifying enzyme genes unique perspective investigated obtain permission directly
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headline:Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening
description:The oil palm (Elaeis guineensis) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2β-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.
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description:The oil palm (Elaeis guineensis) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2β-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.
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