
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Quantitative analysis of tRNA modifications by HPLC-coupled mass spectrometry - PMC
Description:
Post-transcriptional modification of RNA is an important determinant of RNA quality control, translational efficiency, RNA-protein interactions, and stress response. This is illustrated by the observation of toxicant-specific changes in the spectrum ...
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Keywords {🔍}
doi, rna, trna, ribonucleosides, google, scholar, pubmed, cat, pmc, modified, article, free, step, modifications, hplc, agilent, total, analysis, sample, mass, cells, cell, min, tube, table, quantification, methods, figure, purification, modification, lcms, time, ribonucleoside, parameters, water, stress, species, critical, protocol, set, data, vial, pellet, approach, run, open, yeast, samples, concentration, column,
Topics {✒️}
dimensional thin-layer chromatography16 du-640 uv-visible spectrophotometer high-performance liquid chromatography helper component-proteinase expression liquid chromatography-mass spectrometry polyacrylamide-free rna oligonucleotides high-performance gel filtration strand-specific 5'-o-methylation uplc-coupled qqq system pmc beta search calculate treatment-induced chances anticipated results high mass-accuracy ms diode array detector mass spectrometry-based methods reversed-phase hplc resolution temperature-controlled column compartment trm9-catalyzed trna modifications add rna-free water 2’-o-methyl ribose moiety 14mm β-mercaptoethanol immediately facilitate systems-level studies rnase-free h2o 5 rnase-free h2o collision-induced dissociation ms ion-exchange chromatography detect 2’-o-ribosyladenosine phosphate rnase-free water [google scholar] 51 15-minute lc-ms run license information pmcid cambridge isotope laboratories publisher site pdf lc-ms/ms analysis altered regulatory pathways rna-free water trm9-dependent ribonucleosides mcm5u multiple reaction monitoring mass spectrometer setup codon-biased translation materials reagents ribonucleosides average fold-change values wild-type yeast exposed agilent qqq systems codon-biased mrna lc-ms systems external calibration curve reversed-phase resolution qqq mass spectrometer blue screw caps
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- How much profit does https://scholar.google.com/scholar_lookup?journal=J%20Chromatogr%20A&title=Studying%20the%20mechanism%20of%20RNA%20separations%20using%20RNA%20chromatography%20and%20its%20application%20in%20the%20analysis%20of%20ribosomal%20RNA%20and%20RNA:RNA%20interactions&author=SP%20Waghmare&author=P%20Pousinis&author=DP%20Hornby&author=MJ%20Dickman&volume=1216&publication_year=2009&pages=1377-1382&pmid=19155018&doi=10.1016/j.chroma.2008.12.077& make?
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- How much does https://scholar.google.com/scholar_lookup?journal=Nucleic%20Acids%20Res&title=A%20multidimensional%20platform%20for%20the%20purification%20of%20non-coding%20RNA%20species&author=YH%20Chionh&volume=41&publication_year=2013&pages=e168&pmid=23907385&doi=10.1093/nar/gkt668& pull in?
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