
DOI . ORG {
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Title[redir]:
Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres | Nature
Description:
The biochemical basis for the regulation of fibre-type determination in skeletal muscle is not well understood. In addition to the expression of particular myofibrillar proteins, type I (slow-twitch) fibres are much higher in mitochondrial content and are more dependent on oxidative metabolism than type II (fast-twitch) fibres1. We have previously identified a transcriptional co-activator, peroxisome-proliferator-activated receptor-γ co-activator-1 (PGC-1α), which is expressed in several tissues including brown fat and skeletal muscle, and that activates mitochondrial biogenesis and oxidative metabolism2,3,4. We show here that PGC-1α is expressed preferentially in muscle enriched in type I fibres. When PGC-1α is expressed at physiological levels in transgenic mice driven by a muscle creatine kinase (MCK) promoter, a fibre type conversion is observed: muscles normally rich in type II fibres are redder and activate genes of mitochondrial oxidative metabolism. Notably, putative type II muscles from PGC-1α transgenic mice also express proteins characteristic of type I fibres, such as troponin I (slow) and myoglobin, and show a much greater resistance to electrically stimulated fatigue. Using fibre-type-specific promoters, we show in cultured muscle cells that PGC-1α activates transcription in cooperation with Mef2 proteins and serves as a target for calcineurin signalling, which has been implicated in slow fibre gene expression. These data indicate that PGC-1α is a principal factor regulating muscle fibre type determination.
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Keywords {🔍}
article, muscle, cas, google, scholar, nature, skeletal, coactivator, type, access, cell, mitochondrial, content, transcriptional, expression, biol, pgcα, fibre, fibres, mice, slow, cookies, biogenesis, physiol, pgc, usa, medical, texas, privacy, data, gene, peroxisome, receptor, open, mol, department, function, formation, lin, olson, proteins, transgenic, kinase, mef, calcineurin, proliferatoractivated, regulates, control, pathway, center,
Topics {✒️}
peroxisome-proliferator-activated receptor-γ nature portfolio permissions reprints privacy policy fast-fibre-type-specific regulatory elements advertising muscle development social media nature 413 nature 418 nature author information authors fibre-type-specific promoters fibre-type-specific transcription regulates mitochondrial biogenesis metachromatic dye-atpase method insulin-sensitive glucose transporter pgc-1α transgenic mice author correspondence calcineurin-dependent pathway pgc-1α activates transcription activator pgc-1α drives activates mitochondrial biogenesis springerlink instant access fibre-type determination exercise-regulated mitochondrial slow-twitch muscle fibres cardiac muscle expression content muscle regulator multiple calcium-regulated signals permissions muscle creatine kinase personal data thermogenic coactivator pgc-1 coactivator pgc-1 cooperates cold-inducible coactivator fibre type conversion transcriptional coactivator pgc-1 mitochondrial content transgenic mice driven generating transgenic mice data protection nuclear receptors linked skeletal muscle slow chen-yu zhang privacy myocyte enhancer factor-2 skeletal muscle elizabeth nuclear signalling networks myoglobin-mutant mice
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headline:Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres
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headline:Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres
description:The biochemical basis for the regulation of fibre-type determination in skeletal muscle is not well understood. In addition to the expression of particular myofibrillar proteins, type I (slow-twitch) fibres are much higher in mitochondrial content and are more dependent on oxidative metabolism than type II (fast-twitch) fibres1. We have previously identified a transcriptional co-activator, peroxisome-proliferator-activated receptor-γ co-activator-1 (PGC-1α), which is expressed in several tissues including brown fat and skeletal muscle, and that activates mitochondrial biogenesis and oxidative metabolism2,3,4. We show here that PGC-1α is expressed preferentially in muscle enriched in type I fibres. When PGC-1α is expressed at physiological levels in transgenic mice driven by a muscle creatine kinase (MCK) promoter, a fibre type conversion is observed: muscles normally rich in type II fibres are redder and activate genes of mitochondrial oxidative metabolism. Notably, putative type II muscles from PGC-1α transgenic mice also express proteins characteristic of type I fibres, such as troponin I (slow) and myoglobin, and show a much greater resistance to electrically stimulated fatigue. Using fibre-type-specific promoters, we show in cultured muscle cells that PGC-1α activates transcription in cooperation with Mef2 proteins and serves as a target for calcineurin signalling, which has been implicated in slow fibre gene expression. These data indicate that PGC-1α is a principal factor regulating muscle fibre type determination.
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