
DOI . ORG {
}
Title[redir]:
Human hepatic stellate cell isolation and characterization | Journal of Gastroenterology
Description:
The hepatic stellate cells (HSCs) localize at the space of Disse in the liver and have multiple functions. They are identified as the major contributor to hepatic fibrosis. Significant understanding of HSCs has been achieved using rodent models and isolated murine HSCs; as well as investigating human liver tissues and human HSCs. There is growing interest and need of translating rodent study findings to human HSCs and human liver diseases. However, species-related differences impose challenges on the translational research. In this review, we focus on the current information on human HSCs isolation methods, human HSCs markers, and established human HSC cell lines.
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Keywords {π}
cells, human, liver, pubmed, google, scholar, article, cas, hscs, hepatic, stellate, cell, expression, hsc, normal, central, rat, fibrosis, culture, isolated, hepatology, growth, studies, isolation, livers, activated, protein, Ξ±sma, reported, desmin, factor, line, lines, droplets, receptor, primary, activation, positive, express, gfap, tissue, identified, observed, lipid, myofibroblasts, characterization, markers, fatstoring, method, fibrotic,
Topics {βοΈ}
b7-h1-mediated t-cell apoptosis raf/fos/jun/egr activation alpha-smooth-muscle-actin-positive cells platelet-derived growth factor monocyte/macrophage-lineage markers alpha-smooth-muscle actin expression article download pdf transmission electron microscopy precision-cut liver slices cell surface-bound protease leptin receptor ob-rl hepatocyte growth factor david allen brenner human hepatic ito-cell Ξ±-smooth muscle actin smooth muscle Ξ±-actin c-type natriuretic peptide post-receptor mechanism independent septum transversum-derived mesothelium fluorescent-activated cell sorting cytoglobin/stellate cell activation human liver physiology gold-chloride impregnation technique intermediate-zonal human hscs stellate-cell growth bile-duct epithelial cells human fat-storing cells epidermal growth factor hepatotoxic-induced liver fibrosis hepatic stellate cell cellular retinol-binding protein-1 retinyl-palmitate lipase activity cultured fat-storing cells vitamin a-storing cells alpha b-crystallin expression cellular retinol-binding proteins immortalized hsc lines published cell lines hepatic stellate cells hepatic stellate cells fluorescence-activated cell sorting stellate cell activation monoclonal primary antibody vitamin a-rich hscs characteristic morphologic feature cell line immortalized tumor microenvironment research fibrogenic cell fate human stellate cells human cell line
Questions {β}
- Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?
- On the origin of stellate cells: mesodermal, endodermal or neuro-ectodermal?
- Whatβs new in liver fibrosis?
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headline:Human hepatic stellate cell isolation and characterization
description:The hepatic stellate cells (HSCs) localize at the space of Disse in the liver and have multiple functions. They are identified as the major contributor to hepatic fibrosis. Significant understanding of HSCs has been achieved using rodent models and isolated murine HSCs; as well as investigating human liver tissues and human HSCs. There is growing interest and need of translating rodent study findings to human HSCs and human liver diseases. However, species-related differences impose challenges on the translational research. In this review, we focus on the current information on human HSCs isolation methods, human HSCs markers, and established human HSC cell lines.
datePublished:2017-11-01T00:00:00Z
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headline:Human hepatic stellate cell isolation and characterization
description:The hepatic stellate cells (HSCs) localize at the space of Disse in the liver and have multiple functions. They are identified as the major contributor to hepatic fibrosis. Significant understanding of HSCs has been achieved using rodent models and isolated murine HSCs; as well as investigating human liver tissues and human HSCs. There is growing interest and need of translating rodent study findings to human HSCs and human liver diseases. However, species-related differences impose challenges on the translational research. In this review, we focus on the current information on human HSCs isolation methods, human HSCs markers, and established human HSC cell lines.
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