We are analyzing https://link.springer.com/article/10.1007/s00418-008-0544-2.

Title:
Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver | Histochemistry and Cell Biology
Description:
Hepatic stellate cells (HSCs) have important roles in the pathogenesis of liver fibrosis and cirrhosis. As response to chronic injury HSCs are activated and change from quiescent into myofibroblast-like cells. Several HSC-specific markers have been described in rat or mouse models. The aim of our work was to identify the best marker(s) for human HSCs. To this end we used the automated high throughput NexES IHC staining device (Ventana Medical Systems) to incubate sections under standardized conditions. Formalin fixed paraffin embedded (FFPE) normal and diseased human livers were studied. With immunohistochemistry we examined the expression of synemin, desmin, vimentin, vinculin, neurotrophin-3 (NT-3), α-smooth muscle actin (α-SMA), cellular retinol-binding protein-1 (CRBP-1), glial fibrillary acidic protein (GFAP), cysteine- and glycine-rich protein 2 (CRP2), and cytoglobin/stellate cell activation-associated protein (cygb/STAP). This is the first study in which a series of HSC markers is compared on serial FFPE human tissues. CRBP-1 clearly stains lobular HSCs without reacting with smooth muscle cells (SMCs) and shows variable cholangiocyte positivity. Vinculin has a similar staining pattern as CRBP-1 but additionally stains SMCs, and (myo)fibroblasts. In conclusion, we therefore propose to use CRBP-1 and/or vinculin to stain HSCs in human liver tissues.
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Keywords {🔍}

article, google, scholar, pubmed, cells, cas, stellate, hepatic, liver, protein, cell, rat, human, van, hepatology, cellular, expression, retinolbinding, normal, roskams, geerts, desmin, muscle, pathol, biol, vinculin, activated, markers, quiescent, rossen, hscs, fibrosis, livers, glial, fibrillary, acidic, privacy, cookies, content, biology, vander, borght, synemin, actin, crbp, access, proteins, ito, perisinusoidal, intermediate,

Topics {✒️}

alpha-smooth-muscle actin expression month download article/chapter α-smooth muscle actin sara vander borght homologously recombined erythopoietin-sv40 schering-plough unconditional grant cellular retinol-binding protein-1 cellular retinol-binding protein fibrotic/cirrhotic human liver vitamin a-storing cells cellular retinol-binding proteins cytoglobin/stellate cell activation fat-storing cells hepatic stellate cells lim-domain protein cysteine experimental liver fibrosis open reading frames smooth muscle cells perisinusoidal stellate cells full article pdf intermediate filament proteins hepatic retinol metabolism privacy choices/manage cookies glycine-rich protein 2 elke van rossen adult human liver human cirrhosis human liver tissues stellate cell activation chronic injury hscs stains lobular hscs fibrotic human livers liver fibrosis diseased human livers human hepatocellular carcinoma vander borght van den bk similar staining pattern signal transduction system antigen fusion gene article histochemistry geerts contributed equally fibroblast activation protein cell-matrix interactions cygb/stap antibodies ventana medical systems eicosanoid-mediated contractility peroxidase activity found extracellular matrix components de bleser pj

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         description:Hepatic stellate cells (HSCs) have important roles in the pathogenesis of liver fibrosis and cirrhosis. As response to chronic injury HSCs are activated and change from quiescent into myofibroblast-like cells. Several HSC-specific markers have been described in rat or mouse models. The aim of our work was to identify the best marker(s) for human HSCs. To this end we used the automated high throughput NexES IHC staining device (Ventana Medical Systems) to incubate sections under standardized conditions. Formalin fixed paraffin embedded (FFPE) normal and diseased human livers were studied. With immunohistochemistry we examined the expression of synemin, desmin, vimentin, vinculin, neurotrophin-3 (NT-3), α-smooth muscle actin (α-SMA), cellular retinol-binding protein-1 (CRBP-1), glial fibrillary acidic protein (GFAP), cysteine- and glycine-rich protein 2 (CRP2), and cytoglobin/stellate cell activation-associated protein (cygb/STAP). This is the first study in which a series of HSC markers is compared on serial FFPE human tissues. CRBP-1 clearly stains lobular HSCs without reacting with smooth muscle cells (SMCs) and shows variable cholangiocyte positivity. Vinculin has a similar staining pattern as CRBP-1 but additionally stains SMCs, and (myo)fibroblasts. In conclusion, we therefore propose to use CRBP-1 and/or vinculin to stain HSCs in human liver tissues.
         datePublished:2008-12-04T00:00:00Z
         dateModified:2008-12-04T00:00:00Z
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            Hepatic stellate cells
            Vinculin
            CRBP-1-immunohistochemistry
            Fibrosis
            Cirrhosis
            Biomedicine
            general
            Cell Biology
            Biochemistry
            Developmental Biology
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      description:Hepatic stellate cells (HSCs) have important roles in the pathogenesis of liver fibrosis and cirrhosis. As response to chronic injury HSCs are activated and change from quiescent into myofibroblast-like cells. Several HSC-specific markers have been described in rat or mouse models. The aim of our work was to identify the best marker(s) for human HSCs. To this end we used the automated high throughput NexES IHC staining device (Ventana Medical Systems) to incubate sections under standardized conditions. Formalin fixed paraffin embedded (FFPE) normal and diseased human livers were studied. With immunohistochemistry we examined the expression of synemin, desmin, vimentin, vinculin, neurotrophin-3 (NT-3), α-smooth muscle actin (α-SMA), cellular retinol-binding protein-1 (CRBP-1), glial fibrillary acidic protein (GFAP), cysteine- and glycine-rich protein 2 (CRP2), and cytoglobin/stellate cell activation-associated protein (cygb/STAP). This is the first study in which a series of HSC markers is compared on serial FFPE human tissues. CRBP-1 clearly stains lobular HSCs without reacting with smooth muscle cells (SMCs) and shows variable cholangiocyte positivity. Vinculin has a similar staining pattern as CRBP-1 but additionally stains SMCs, and (myo)fibroblasts. In conclusion, we therefore propose to use CRBP-1 and/or vinculin to stain HSCs in human liver tissues.
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         Hepatic stellate cells
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         CRBP-1-immunohistochemistry
         Fibrosis
         Cirrhosis
         Biomedicine
         general
         Cell Biology
         Biochemistry
         Developmental Biology
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