We are analyzing https://link.springer.com/article/10.1007/bf00194608.

Title:
Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver | Virchows Archiv
Description:
To identify Ito cells in normal and pathological adult human livers, immunohistochemical studies were performed by the avidin-biotin-peroxidase complex method using monoclonal antibodies for α-smooth muscle actin (ASMA), desmin, and vimentin. Fifty one needle biopsies, 7 surgically resected specimens, and 5 autopsy specimens were studied. In the normal adult liver vascular smooth muscle cells and pericytes, together with perisinusoidal cells with thin cytoplasmic processes were positive for ASMA. These latter cells formed a loose and discontinuous layer along the sinusoidal walls. Immunoelectron microscopy showed that the ASMA-positive perisinusoidal cells were Ito cells containing fat droplets. The other sinusoidal lining cells were negative for ASMA. In chronic liver disease, ASMA-positive Ito cells showed an increase in number, size, and the intensity of immunostaining in areas of piecemeal necrosis), and formed a continuous cellular network. These cells were dendritic in shape with irregularly elongated cytoplasmic processes and contained an increased amount of microfilaments, in association with loss of the characteristic fat droplets. Thus, their ultrastructural features corresponded to those of myofibroblastic cells. Ito cells showed no staining for desmin in both normal and pathological livers. These results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers.
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Keywords {🔍}

cells, google, scholar, liver, ito, normal, human, article, muscle, cell, actin, livers, access, adult, desmin, perisinusoidal, hepatic, gabbiani, privacy, cookies, content, virchows, identification, enzan, hara, himeno, αsmooth, fibrosis, fatstoring, pathol, publish, research, search, immunohistochemical, iwamura, onishi, yamamoto, smooth, sinusoidal, chronic, open, stellate, arch, hepatology, skalli, kochi, data, information, log, journal,

Topics {✒️}

α-smooth-muscle-actin-positive cells α-smooth-muscle actin expression avidin-biotin-peroxidase complex method α-smooth muscle actin month download article/chapter vitamin a-storing cells asma-positive perisinusoidal cells smooth muscle cells adult human liver pathological smooth-muscle adult human livers smooth muscle differentiation perisinusoidal stellate cells privacy choices/manage cookies full article pdf actin-isoform pattern hepatic sinusoidal wall kupffer cells ito cells showed related subjects focal hepatic injury fat-storing cell fat-storing cells human hepatocellular carcinoma human cirrhotic nodules sinusoidal lining cells alcoholic liver injury experimental hepatic fibrosis identify ito cells transformed ito cells thin cytoplasmic processes diseased human livers fibrotic human livers normal human liver specific plasma cells experimental liver fibrosis lymphoreticular cell foundation isolated hepatic lipocytes chronic liver disease immunoelectron microscopy showed european economic area scope submit manuscript continuous cellular network check access immunological micro-milieu anti-synthetase syndrome comprehensive autoantigen-ome focal nodular hyperplasia perfusion fixation technique silver-impregnation methods

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         headline:Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver
         description:To identify Ito cells in normal and pathological adult human livers, immunohistochemical studies were performed by the avidin-biotin-peroxidase complex method using monoclonal antibodies for α-smooth muscle actin (ASMA), desmin, and vimentin. Fifty one needle biopsies, 7 surgically resected specimens, and 5 autopsy specimens were studied. In the normal adult liver vascular smooth muscle cells and pericytes, together with perisinusoidal cells with thin cytoplasmic processes were positive for ASMA. These latter cells formed a loose and discontinuous layer along the sinusoidal walls. Immunoelectron microscopy showed that the ASMA-positive perisinusoidal cells were Ito cells containing fat droplets. The other sinusoidal lining cells were negative for ASMA. In chronic liver disease, ASMA-positive Ito cells showed an increase in number, size, and the intensity of immunostaining in areas of piecemeal necrosis), and formed a continuous cellular network. These cells were dendritic in shape with irregularly elongated cytoplasmic processes and contained an increased amount of microfilaments, in association with loss of the characteristic fat droplets. Thus, their ultrastructural features corresponded to those of myofibroblastic cells. Ito cells showed no staining for desmin in both normal and pathological livers. These results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers.
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      headline:Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver
      description:To identify Ito cells in normal and pathological adult human livers, immunohistochemical studies were performed by the avidin-biotin-peroxidase complex method using monoclonal antibodies for α-smooth muscle actin (ASMA), desmin, and vimentin. Fifty one needle biopsies, 7 surgically resected specimens, and 5 autopsy specimens were studied. In the normal adult liver vascular smooth muscle cells and pericytes, together with perisinusoidal cells with thin cytoplasmic processes were positive for ASMA. These latter cells formed a loose and discontinuous layer along the sinusoidal walls. Immunoelectron microscopy showed that the ASMA-positive perisinusoidal cells were Ito cells containing fat droplets. The other sinusoidal lining cells were negative for ASMA. In chronic liver disease, ASMA-positive Ito cells showed an increase in number, size, and the intensity of immunostaining in areas of piecemeal necrosis), and formed a continuous cellular network. These cells were dendritic in shape with irregularly elongated cytoplasmic processes and contained an increased amount of microfilaments, in association with loss of the characteristic fat droplets. Thus, their ultrastructural features corresponded to those of myofibroblastic cells. Ito cells showed no staining for desmin in both normal and pathological livers. These results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers.
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      dateModified:
      pageStart:249
      pageEnd:256
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         Adult human liver
         Ito cell
         Myofibroblast
         Immunocytochemistry
         α-Smooth muscle actin
         Pathology
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