
DOI . ORG {
}
Title[redir]:
Emerging Technologies for CTC Detection Based on Depletion of Normal Cells | SpringerLink
Description:
Properly conducted, an enrichment step can improve selectivity, sensitivity, yield, and most importantly, significantly reduce the time needed to isolate rare circulating tumor cells (CTCs). The enrichment process can be broadly categorized as positive selection...
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Keywords {🔍}
google, scholar, cancer, cells, pubmed, cas, article, breast, cell, circulating, tumor, blood, res, patients, detection, enrichment, clin, chapter, zborowski, transition, human, chalmers, magnetic, neck, epithelial, mesenchymal, depletion, rare, carcinoma, head, stem, negative, immunomagnetic, separation, flow, pantel, quadrupole, tumour, peripheral, metastatic, epithelialmesenchymal, privacy, cookies, content, information, publish, research, search, residual, disease,
Topics {✒️}
human colon-cancer-initiating cells month download article/chapter human breast cancer–observations cell search system epithelial-mesenchymal transition markers minimal residual disease magnetophoretic cell sorting device instant download privacy choices/manage cookies immunomagnetic cell enrichment negative selection method disseminated tumor cells human breast cancer quadrupole magnetic field antibody binding capacity positive selection methodology editor information editors magnetic cell separation stem cell markers breast tumor cells rare-cell detector disseminating tumour cells circulating epithelial cells epithelial mesenchymal transition epithelial-mesenchymal transition ehrlich ascites tumour cytokeratin-positive cells cell tracking velocimetry metastatic breast cancer iii breast cancer breast cancer relates circulating tumor cells mesenchymal tumor cells squamous cell carcinoma ohio state university immunomagnetic cell separation rare cancer cells immune-induced epithelial high speed detection rare cell isolation tumor-antigen heterogeneity hematopoietic progenitor cells specific biological properties k-ras genes miltenyi biotec gmbh pulse-injection studies magnetically induced motion superparamagnetic microparticles detected institut jules bordet normal cells chapter
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ScholarlyArticle:
headline:Emerging Technologies for CTC Detection Based on Depletion of Normal Cells
pageEnd:110
pageStart:97
image:https://media.springernature.com/w153/springer-static/cover/book/978-3-642-28160-0.jpg
genre:
Medicine
Medicine (R0)
isPartOf:
name:Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer
isbn:
978-3-642-28160-0
978-3-642-28159-4
type:Book
publisher:
name:Springer Berlin Heidelberg
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Maryam Lustberg
affiliation:
name:The Ohio State University
address:
name:Internal Medicine, Division of Medical Oncology, The Ohio State University, Columbus, USA
type:PostalAddress
type:Organization
type:Person
name:Kris R. Jatana
affiliation:
name:The Ohio State University and Nationwide Children’s Hospital
address:
name:Department of Otolaryngology—Head and Neck Surgery, The Ohio State University and Nationwide Children’s Hospital, Columbus, USA
type:PostalAddress
type:Organization
type:Person
name:Maciej Zborowski
affiliation:
name:Cleveland Clinic
address:
name:Department of Biomedical Engineering, Cleveland Clinic, Cleveland, USA
type:PostalAddress
type:Organization
type:Person
name:Jeffrey J. Chalmers
affiliation:
name:The Ohio State University
address:
name:Professor William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Triple Negative Breast Cancer, Disseminate Tumor Cell, CellSearch System, Cytokeratin Positive Cell, Metastatic Triple Negative Breast Cancer
description:Properly conducted, an enrichment step can improve selectivity, sensitivity, yield, and most importantly, significantly reduce the time needed to isolate rare circulating tumor cells (CTCs). The enrichment process can be broadly categorized as positive selection versus negative depletion, or in some cases, a combination of both. We have developed a negative depletion CTC enrichment strategy that relies on the removal of normal cells using immunomagnetic separation in the blood of cancer patients. This method is based on the combination of magnetic and fluid forces in an axial, laminar flow in long cylinders placed in quadrupole magnets. Using this technology, we have successfully isolated CTCs from patients with breast carcinoma and squamous cell carcinoma of the head and neck. In contrast to a positive selection methodology, this approach provides an unbiased characterization of these cells, including markers associated with epithelial mesenchymal transition.
datePublished:2012
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isbn:
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logo:
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name:The Ohio State University
address:
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name:Cleveland Clinic
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type:PostalAddress
type:Organization
name:Kris R. Jatana
affiliation:
name:The Ohio State University and Nationwide Children’s Hospital
address:
name:Department of Otolaryngology—Head and Neck Surgery, The Ohio State University and Nationwide Children’s Hospital, Columbus, USA
type:PostalAddress
type:Organization
name:Maciej Zborowski
affiliation:
name:Cleveland Clinic
address:
name:Department of Biomedical Engineering, Cleveland Clinic, Cleveland, USA
type:PostalAddress
type:Organization
name:Jeffrey J. Chalmers
affiliation:
name:The Ohio State University
address:
name:Professor William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, USA
type:PostalAddress
type:Organization
email:[email protected]
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name:Internal Medicine, Division of Medical Oncology, The Ohio State University, Columbus, USA
name:Department of Otolaryngology—Head and Neck Surgery, The Ohio State University and Nationwide Children’s Hospital, Columbus, USA
name:Department of Biomedical Engineering, Cleveland Clinic, Cleveland, USA
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