We are analyzing https://link.springer.com/article/10.1186/bcr2333.

Title:
Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients | Breast Cancer Research
Description:
Introduction The persistence of circulating tumor cells (CTC) in breast cancer patients might be associated with stem cell like tumor cells which have been suggested to be the active source of metastatic spread in primary tumors. Furthermore, these cells also may undergo phenotypic changes, known as epithelial-mesenchymal transition (EMT), which allows them to travel to the site of metastasis formation without getting affected by conventional treatment. Here we evaluated 226 blood samples of 39 metastatic breast cancer patients during a follow-up of palliative chemo-, antibody – or hormonal therapy for the expression of the stem cell marker ALDH1 and markers for EMT and correlated these findings with the presence of CTC and response to therapy. Methods 2 × 5 ml blood was analyzed for CTC with the AdnaTest BreastCancer (AdnaGen AG) for the detection of EpCAM, MUC-1 and HER2 transcripts. The recovered c-DNA was additionally multiplex tested for three EMT markers [Twist1, Akt2, PI3Kα] and separately for the tumor stem-cell markers ALDH1. The identification of EMT markers was considered positive if at least one marker was detected in the sample. Results 97% of 30 healthy donor samples investigated were negative for EMT and 95% for ALDH1 transcripts. CTC were detected in 69/226 (31%) cancer samples. In the CTC (+) group, 62% were positive for at least one of the EMT markers and 69% for ALDH1, respectively. In the CTC (-) group the percentages were 7% and 14%, respectively. In non-responders, EMT and ALDH1 expression was found in 62% and 44% of patients, in responders the rates were 10% and 5%, respectively. Conclusions Our data indicate that a major proportion of CTC of metastatic breast cancer patients shows EMT and tumor stem cell characteristics. Further studies are needed to prove whether these markers might serve as an indicator for therapy resistant tumor cell populations and, therefore, an inferior prognosis.
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cancer, breast, cells, tumor, patients, emt, stem, cell, markers, article, pubmed, google, scholar, aldh, metastatic, samples, ctcs, therapy, ctc, blood, cas, response, metastasis, treatment, expression, res, primary, akt, detected, data, circulating, positive, group, patient, marker, twist, analysis, nonresponders, responders, disease, phenotype, bone, figure, research, detection, study, marrow, pcr, ngμl, transition,

Topics {✒️}

sabine kasimir-bauer egfr/her2/pi3k/akt pathway article download pdf epithelial-mesenchymal transition markers phosphoinositide-dependent protein kinase-1 breast cancer-related therapies tumorigenic breast-cancer cells therapy-resistant cell populations pi3k/akt signaling kinases epithelial-mesenchymal transition epithelial mesenchymal transition tumor cell enrichment/selection signal-transduction pathways activated cell stem cell akt2 ser/thr kinase epidermal/mesenchymal transition full size image stem cell population cd44+/cd24-/low cells cancer stem cells epithelial-mesenchymal transitions stem cell properties stem cell preparations stem cell rev metastatic breast cancer ductal breast cancer stem cell markers tumor stem cells tumour progression metastatic cancer cells kasimir-bauer tumor cell growth breast cancer cells privacy choices/manage cookies authors’ original file epithelial cell homeostasis invasive breast carcinoma breast cancer res signal-transduction pathways exclude false-positive results cd44+/cd24- phenotype correlated breast cancer subtypes recurrent breast cancer emt multiplex rt-pcr negative prognostic impact early breast cancer circulating tumor cells human breast cancers article number r46 breast cancer patients

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Polyak K: Breast cancer stem cells: a case of mistaken identity?

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         headline:Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients
         description:The persistence of circulating tumor cells (CTC) in breast cancer patients might be associated with stem cell like tumor cells which have been suggested to be the active source of metastatic spread in primary tumors. Furthermore, these cells also may undergo phenotypic changes, known as epithelial-mesenchymal transition (EMT), which allows them to travel to the site of metastasis formation without getting affected by conventional treatment. Here we evaluated 226 blood samples of 39 metastatic breast cancer patients during a follow-up of palliative chemo-, antibody – or hormonal therapy for the expression of the stem cell marker ALDH1 and markers for EMT and correlated these findings with the presence of CTC and response to therapy. 2 × 5 ml blood was analyzed for CTC with the AdnaTest BreastCancer (AdnaGen AG) for the detection of EpCAM, MUC-1 and HER2 transcripts. The recovered c-DNA was additionally multiplex tested for three EMT markers [Twist1, Akt2, PI3Kα] and separately for the tumor stem-cell markers ALDH1. The identification of EMT markers was considered positive if at least one marker was detected in the sample. 97% of 30 healthy donor samples investigated were negative for EMT and 95% for ALDH1 transcripts. CTC were detected in 69/226 (31%) cancer samples. In the CTC (+) group, 62% were positive for at least one of the EMT markers and 69% for ALDH1, respectively. In the CTC (-) group the percentages were 7% and 14%, respectively. In non-responders, EMT and ALDH1 expression was found in 62% and 44% of patients, in responders the rates were 10% and 5%, respectively. Our data indicate that a major proportion of CTC of metastatic breast cancer patients shows EMT and tumor stem cell characteristics. Further studies are needed to prove whether these markers might serve as an indicator for therapy resistant tumor cell populations and, therefore, an inferior prognosis.
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