We are analyzing https://link.springer.com/article/10.1007/s10549-007-9771-9.

Title:
The significance of circulating epithelial cells in Breast Cancer patients by a novel negative selection method | Breast Cancer Research and Treatment
Description:
Purpose: A negative selection method for the enumeration and characterization of circulating epithelial/cancer cells (CCC) in Breast Cancer (BC) patients is described. This manual procedure yields reproducible results of high sensitivity and selectivity suitable for research laboratories. Patients and methods: We conducted a prospective blood sampling study in 105 women with stage 1–4 BC attending clinics at the University of Maryland Greenebaum Cancer Center to define the prevalence of CCC utilizing our sensitive double gradient centrifugation and magnetic cell sorting CCC detection and enumeration method. CCC were isolated and enumerated from 15 to 20 ml of venous blood drawn before the start of systemic therapy and periodically thereafter for up to 24 months. One or more CCC/sample was considered a positive result. Results: We analyzed 487samples for the presence of CCC; the median number of samples/patient was 4 (range 1–8). CCC were detected in 56% of patients, 19%—stage 1; 43%—stage 2; 46%—stage 3; 83%—stage 4. The probability of being positive for the presence of CCC is significantly associated with the stage of cancer (P < 0.0001). The frequency of CCC positive patients and samples increased with the advancing stage of disease. Presence of more than 10 CCC/sample was associated with the decreased survival and increased probability of having metastatic disease P = 0.001. Conclusions: Increasing number of CCC/sample correlates with the adverse outcome and poorer survival (P < 0.0001). Our CCC test based on the negative selection procedure may provide valuable prognostic information.
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

cancer, cells, article, breast, circulating, pubmed, google, scholar, patients, tumor, cas, ccc, blood, stage, metastatic, clin, res, epithelial, detection, survival, access, research, method, privacy, cookies, content, information, negative, selection, disease, usa, publish, search, treatment, clinical, tkaczuk, positive, open, med, oncol, author, data, log, journal, significance, december, katherine, rak, goloubeva, tait,

Topics {✒️}

month download article/chapter negative selection method circulating epithelial/cancer cells negative selection procedure real-world clinical practice circulating cytokeratin-positive cells metastatic breast cancer metastatic breast cancer related subjects breast cancer cells circulating epithelial cells full article pdf circulating tumor cells breast cancer patients privacy choices/manage cookies disseminated tumor cells tumor cells circulate zhao-ping lum cells predict survival baseline circulating stem cytokeratin 19-positive cells author information authors treatment article breast cancer venous blood drawn european economic area low proliferative activity multimolecular marker assay estrogen-receptor status van echo & paul 42nd annual meeting ccc diagnostics llc conditions privacy policy bone marrow micrometastases check access instant access ccc test based epithelial cells survival access accepting optional cookies article log faye feldman ming tan prostate cancer ccc/sample correlates article tkaczuk healthy subjects clinical stage cancer res 61 journal finder publish

Questions {❓}

Braun S, Marth C (2004) Circulating tumor cells in metastatic breast cancer—toward individualized treatment?
Muller V, Pantel K (2005) BM micrometastases and circulating tumor cells in breast cancer patients: where have we been, where are we now and where does the future lie?

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         headline:The significance of circulating epithelial cells in Breast Cancer patients by a novel negative selection method
         description: Purpose: A negative selection method for the enumeration and characterization of circulating epithelial/cancer cells (CCC) in Breast Cancer (BC) patients is described. This manual procedure yields reproducible results of high sensitivity and selectivity suitable for research laboratories. Patients and methods: We conducted a prospective blood sampling study in 105 women with stage 1–4 BC attending clinics at the University of Maryland Greenebaum Cancer Center to define the prevalence of CCC utilizing our sensitive double gradient centrifugation and magnetic cell sorting CCC detection and enumeration method. CCC were isolated and enumerated from 15 to 20 ml of venous blood drawn before the start of systemic therapy and periodically thereafter for up to 24 months. One or more CCC/sample was considered a positive result. Results: We analyzed 487samples for the presence of CCC; the median number of samples/patient was 4 (range 1–8). CCC were detected in 56% of patients, 19%—stage 1; 43%—stage 2; 46%—stage 3; 83%—stage 4. The probability of being positive for the presence of CCC is significantly associated with the stage of cancer (P < 0.0001). The frequency of CCC positive patients and samples increased with the advancing stage of disease. Presence of more than 10 CCC/sample was associated with the decreased survival and increased probability of having metastatic disease P = 0.001. Conclusions: Increasing number of CCC/sample correlates with the adverse outcome and poorer survival (P < 0.0001). Our CCC test based on the negative selection procedure may provide valuable prognostic information.
         datePublished:2007-12-07T00:00:00Z
         dateModified:2007-12-07T00:00:00Z
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         pageEnd:364
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            Stage 1-4
            Circulating epithelial cells
            Negative selection method
            Prospective blood sampling study
            Association of numbers of epithelial cells with survival
            Oncology
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      headline:The significance of circulating epithelial cells in Breast Cancer patients by a novel negative selection method
      description: Purpose: A negative selection method for the enumeration and characterization of circulating epithelial/cancer cells (CCC) in Breast Cancer (BC) patients is described. This manual procedure yields reproducible results of high sensitivity and selectivity suitable for research laboratories. Patients and methods: We conducted a prospective blood sampling study in 105 women with stage 1–4 BC attending clinics at the University of Maryland Greenebaum Cancer Center to define the prevalence of CCC utilizing our sensitive double gradient centrifugation and magnetic cell sorting CCC detection and enumeration method. CCC were isolated and enumerated from 15 to 20 ml of venous blood drawn before the start of systemic therapy and periodically thereafter for up to 24 months. One or more CCC/sample was considered a positive result. Results: We analyzed 487samples for the presence of CCC; the median number of samples/patient was 4 (range 1–8). CCC were detected in 56% of patients, 19%—stage 1; 43%—stage 2; 46%—stage 3; 83%—stage 4. The probability of being positive for the presence of CCC is significantly associated with the stage of cancer (P < 0.0001). The frequency of CCC positive patients and samples increased with the advancing stage of disease. Presence of more than 10 CCC/sample was associated with the decreased survival and increased probability of having metastatic disease P = 0.001. Conclusions: Increasing number of CCC/sample correlates with the adverse outcome and poorer survival (P < 0.0001). Our CCC test based on the negative selection procedure may provide valuable prognostic information.
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      dateModified:2007-12-07T00:00:00Z
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         Breast Cancer
         Stage 1-4
         Circulating epithelial cells
         Negative selection method
         Prospective blood sampling study
         Association of numbers of epithelial cells with survival
         Oncology
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