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Title:
Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge | Scientific Reports
Description:
Vaccines based on live attenuated Chlamydia elementary bodies (EBs) can cause disease in vaccinated animals and the comparably safer inactivated whole EBs are only marginally protective. Recent studies show that a vaccine formulation comprising UV-inactivated EBs (EB) and appropriate mucosal delivery systems and/or adjuvants induced significant protective immunity. We tested the hypothesis that intranasal delivery of UV-inactivated C. psittaci EB formulated in Vibrio cholerae ghosts (VCG)-chitosan nanoparticles will induce protective immunity against intranasal challenge in SPF chickens. We first compared the impact of VCG and CpG adjuvants on protective immunity following IN mucosal and IM systemic delivery of EB formulated in chitosan hydrogel/microspheres. Immunologic analysis revealed that IN immunization in the presence of VCG induced higher levels of IFN-γ response than IM delivery or the CpG adjuvanted groups. Also, vaccine efficacy evaluation showed enhanced pharyngeal bacterial clearance and protection against lung lesions with the VCG adjuvanted vaccine formulation, thereby establishing the superior adjuvanticity of VCG over CpG. We next evaluated the impact of different concentrations of VCG on protective immunity following IN mucosal immunization. Interestingly, the adjuvanticity of VCG was concentration-dependent, since protective immunity induced following IN mucosal immunization showed dose-dependent immune responses and protection. These studies reveal that formulation of inactivated chlamydial antigens with adjuvants, such as VCG and chitosan increases their ability to induce protective immune responses against challenge.
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nature portfolio privacy policy fluorescence-labeled anti-momp monoclonal-antibody nature ifn-{gamma}/interleukin-17 double-positive cd4+ advertising pcdna1/momp-transfected cos7 cells social media gel + eb + vcg100 formulation showing gel + eb + vcg100 formulation elicited gel + eb + vcg100 vaccine formulation biotin-conjugated antibody specific author information authors solutions reprints gel + eb + vcg35 vaccine formulation t-cell-mediated immune regulation essentially involves introduction commercial elisa kits protein e-mediated lysis gel + eb + vcg35 vaccine group momp-specific antibody titers ifn-gamma-mediated inhibition mannose-conjugated chitosan nanoparticle gel + eb + vcg100 immunized chickens anti-momp antibody titers unbound avidin-enzyme reagent tio2-sol-gel reservoirs original author th1-type cytokines ifn-γ antigen-specific proliferative responses gel + eb + vcg100 afforded momp-based antibody elisa potent anti-inflammatory cytokine antigen-specific cytokine responses significantly elevated ifn-γ gel + eb + vcg100 titers antigen-specific proliferative response vibrio cholerae ghost full size image gel + eb + cpg vaccine group gel + eb + vcg35 vaccine mas-nmr spectroscopy study vcg-adjuvanted vaccine formulation muridarum genital infection66 muridarum subunit antigens gel + eb + cpg immunized chickens �cell‐deficient mice depleted nonspecific binding sites permissions
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- Studies in knockout mice reveal that anti-chlamydial protection requires TH1 cells producing IFN-gamma: is this true for human?
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headline:Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge
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headline:Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge
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