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Title:
Probing Lipid Peroxidation in Ferroptosis: Emphasizing the Utilization of C11-BODIPY-Based Protocols | SpringerLink
Description:
Ferroptosis is a form of regulated cell death that relies on iron and is characterized by the accumulation of lipid peroxides, resulting in oncotic cell swelling and eventual disruption of cellular membranes. Lipid peroxidation, a hallmark of ferroptosis, refers to...
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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Probing Lipid Peroxidation in Ferroptosis: Emphasizing the Utilization of C11-BODIPY-Based Protocols
      pageEnd:72
      pageStart:61
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-0716-3433-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Ferroptosis
         isbn:
            978-1-0716-3433-2
            978-1-0716-3432-5
         type:Book
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Zhangshuai Dai
            affiliation:
                  name:Jinan University
                  address:
                     name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wanting Zhang
            affiliation:
                  name:Jinan University
                  address:
                     name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Liqun Zhou
            affiliation:
                  name:Jinan University
                  address:
                     name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Junqi Huang
            affiliation:
                  name:Jinan University
                  address:
                     name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Ferroptosis, Lipid peroxidation, C11-BODIPY, Flow cytometry, Fluorescence microscopy
      description:Ferroptosis is a form of regulated cell death that relies on iron and is characterized by the accumulation of lipid peroxides, resulting in oncotic cell swelling and eventual disruption of cellular membranes. Lipid peroxidation, a hallmark of ferroptosis, refers to the oxidative deterioration of lipids that contain carbon-carbon double bonds, particularly polyunsaturated fatty acids (PUFAs). Understanding the molecular mechanisms underlying the interplay between ferroptosis and lipid peroxidation and identifying reliable techniques for assessing lipid peroxidation levels are crucial for further advancements in this field of research. Various methods have been developed to detect lipid peroxidation levels, including C11-BODIPY (BODIPY™ 581/591 C11), liperfluo, 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), Click-iT LAA (linoleamide alkyne), and liquid chromatography-mass spectrometry (LC-MS)-based epilipidomics (redox lipidomics). Currently, one of the most commonly used and effective methods is the C11-BODIPY assay, which utilizes a fluorescent probe that selectively sensitizes lipid peroxidation in cell membranes. Incorporating advanced techniques such as flow cytometry and fluorescence microscopy with C11-BODIPY dye is essential for accurate assessment of lipid peroxidation levels in ferroptosis. This chapter aims to provide comprehensive experimental protocols for detecting lipid peroxidation levels indicative of ferroptosis using C11-BODIPY staining and subsequent detection via flow cytometry and fluorescence microscopy.
      datePublished:2023
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         978-1-0716-3432-5
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      name:Jinan University
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      address:
         name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
         type:PostalAddress
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      address:
         name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
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         name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
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      name:Zhangshuai Dai
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            address:
               name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Wanting Zhang
      affiliation:
            name:Jinan University
            address:
               name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Liqun Zhou
      affiliation:
            name:Jinan University
            address:
               name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Junqi Huang
      affiliation:
            name:Jinan University
            address:
               name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
               type:PostalAddress
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      name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
      name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
      name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
      name:Key Laboratory for Regenerative Medicine, Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China
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