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We are analyzing https://www.nature.com/articles/s41594-018-0106-9.

Title:
Terminal uridylyltransferases target RNA viruses as part of the innate immune system | Nature Structural & Molecular Biology
Description:
RNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. Here, we performed a large-scale screen using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), and we identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3ʹ end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3ʹ-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism. A screen for C. elegans antiviral-defense genes identifies a homolog of the mammalian TUT4(7) terminal uridylyltransferase genes and leads to the discovery of 3′-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

pubmed, article, google, scholar, cas, central, viral, data, nature, rna, elegans, orv, infection, supplementary, figure, source, caenorhabditis, cde, cell, expression, antiviral, access, biology, cambridge, mrna, stress, sensor, molecular, genome, independent, uridylation, university, content, viruses, pen, virus, days, research, mutants, gfp, information, domenico, miska, rnas, gene, type, biol, bioinformatics, cookies, structural,

Topics {✒️}

homology-directed genome editing nature portfolio journals permissions reprints nature portfolio strand-specific real-time rt-pcr privacy policy multiple small-rna-mediated pathways cancer research uk caenorhabditis elegans development advertising european research council stem cell research social media library size high-throughput-sequencing support endogenous 22g-rnas abundance nature+ nature 472 nature 548 nature 421 nature high-throughput sequencing reads crispr-cas9 ribonucleoprotein complexes anti-viral immune pathways author correspondence molecular biology mouse biology unit innate immune system functional proteomics reveals springerlink instant access antiviral small rnas tut4/7-mediated uridylation permissions infected individuals exhibited target viral rna tut7 terminal uridylyltransferases terminal uridylyltransferases tut4 inter-individual transmission content u-tail published maps inhibit interferon expression rna-seq data interferon antiviral response vivo rna fish dexh-box helicase eukaryotic rna viruses viral stress sensor tomás di domenico structural basis privacy

Questions {❓}

  • Is anti-viral defence the evolutionary origin of mRNA turnover?

Schema {🗺️}

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      headline:Terminal uridylyltransferases target RNA viruses as part of the innate immune system
      description:RNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. Here, we performed a large-scale screen using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), and we identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3ʹ end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3ʹ-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism. A screen for C. elegans antiviral-defense genes identifies a homolog of the mammalian TUT4(7) terminal uridylyltransferase genes and leads to the discovery of 3′-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism.
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         RNA quality control
         Virology
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         Protein Structure
         Membrane Biology
         Biological Microscopy
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