We are analyzing https://link.springer.com/article/10.1186/s12920-023-01480-4.

Title:
Identification of potential ferroptosis hub genes in acute-on-chronic liver failure based on bioinformatics analysis and experimental verification | BMC Medical Genomics
Description:
Background Ferroptosis plays an important role in the development of acute-on-chronic liver failure (ACLF). The present project aimed to identify and validate the potential ferroptosis-related genes in ACLF by bioinformatics analysis and experimental verification. Materials and methods The GSE139602 dataset was obtained from the Gene Expression Omnibus database and intersected with ferroptosis genes. Ferroptosis-related differentially expressed genes (DEGs) between the ACLF tissue and healthy group were analyzed using bioinformatics methods. Analysis of enrichment, protein‒protein interactions, and hub genes was conducted. Potential drugs targeting these hub genes were retrieved from the DrugBank database. Finally, we performed real-time quantitative PCR (RT-qPCR) to validate the expression of the hub genes. Results A total of 35 ferroptosis-related DEGs were screened, which were enriched in the biosynthesis of amino acids, peroxisomes, fluid shear stress and atherosclerosis. PPI network analysis indicated five ferroptosis-related hub genes, namely, HRAS, TXNRD1, NQO1, PSAT1, and SQSTM1. The experimental validation indicated that the expression levels of HRAS, TXNRD1, NQO1, and SQSTM1 were lower, while the expression level of PSAT1 was higher in ACLF model rats than in healthy rats. Conclusions Our findings reveal that PSAT1, TXNRD1, HRAS, SQSTM1 and NQO1 may affect the development of ACLF by regulating ferroptotic events. These results provide a valid reference for potential mechanisms and identification in ACLF.
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Keywords {🔍}

pubmed, article, genes, ferroptosis, google, scholar, cas, liver, aclf, analysis, degs, cell, gene, expression, hub, ferroptosisrelated, psat, fig, pathway, txnrd, central, cancer, enrichment, acids, database, sqstm, amino, hras, failure, biosynthesis, cysteine, nqo, cells, size, pathways, group, rats, ros, study, full, drugs, death, iron, lipid, involved, levels, table, data, stress, kegg,

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3-triazol-4-yl]-n-isopropyl-n-methyl-3 hsa-mir-26a-3p/epha2 axis performed real-time quantitative pcr org/packages/release/bioc/html/clusterprofer p62-keap1-nrf2 pathway protects user-uploaded gene/measurement sets keap1/nrf2/nqo1 signaling pathway full size image article download pdf alcohol-induced cell death es/tools/venny/index chemical carcinogenesis-dna adducts γ-glutamyl transpeptidase architecture dithiol/disulfide bond balance high short-term mortality growth-arrest-specific factor high-speed tissue grinder oxidative stress-related biomarkers 600 mg/kg d-galactosamine inhibits intra-golgi transport nrf2/nqo1 signaling pathway nrf2-regulated genes nqo1 drug metabolism-cytochrome p450 nrf2-nqo1 axis represents rt‒qpcr results showed customizable protein-protein networks phosphate-buffered saline solution biochim biophys acta dominant-negative regulatory mechanism differentially expressed genes top10 ferroptosis-related degs gpx4 inhibitor-induced ferroptosis liver hepatocellular carcinoma potential ferroptosis-related genes guangdong medical university cytochrome p450 oxidoreductase oncogenic ras promotes identifying ferroptosis-related degs gene expression profiles ferroptosis-related hub genes maintaining cell survival hepatocellular carcinoma cells sqstm1 droplets-based autophagy privacy choices/manage cookies cytochrome p450 pathway full access induced lipid peroxidation cytochrome p450 reductase results ferroptosis-related degs cml cells induced

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Can selenoenzymes resist electrophilic modification?

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         description:Ferroptosis plays an important role in the development of acute-on-chronic liver failure (ACLF). The present project aimed to identify and validate the potential ferroptosis-related genes in ACLF by bioinformatics analysis and experimental verification. The GSE139602 dataset was obtained from the Gene Expression Omnibus database and intersected with ferroptosis genes. Ferroptosis-related differentially expressed genes (DEGs) between the ACLF tissue and healthy group were analyzed using bioinformatics methods. Analysis of enrichment, protein‒protein interactions, and hub genes was conducted. Potential drugs targeting these hub genes were retrieved from the DrugBank database. Finally, we performed real-time quantitative PCR (RT-qPCR) to validate the expression of the hub genes. A total of 35 ferroptosis-related DEGs were screened, which were enriched in the biosynthesis of amino acids, peroxisomes, fluid shear stress and atherosclerosis. PPI network analysis indicated five ferroptosis-related hub genes, namely, HRAS, TXNRD1, NQO1, PSAT1, and SQSTM1. The experimental validation indicated that the expression levels of HRAS, TXNRD1, NQO1, and SQSTM1 were lower, while the expression level of PSAT1 was higher in ACLF model rats than in healthy rats. Our findings reveal that PSAT1, TXNRD1, HRAS, SQSTM1 and NQO1 may affect the development of ACLF by regulating ferroptotic events. These results provide a valid reference for potential mechanisms and identification in ACLF.
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      description:Ferroptosis plays an important role in the development of acute-on-chronic liver failure (ACLF). The present project aimed to identify and validate the potential ferroptosis-related genes in ACLF by bioinformatics analysis and experimental verification. The GSE139602 dataset was obtained from the Gene Expression Omnibus database and intersected with ferroptosis genes. Ferroptosis-related differentially expressed genes (DEGs) between the ACLF tissue and healthy group were analyzed using bioinformatics methods. Analysis of enrichment, protein‒protein interactions, and hub genes was conducted. Potential drugs targeting these hub genes were retrieved from the DrugBank database. Finally, we performed real-time quantitative PCR (RT-qPCR) to validate the expression of the hub genes. A total of 35 ferroptosis-related DEGs were screened, which were enriched in the biosynthesis of amino acids, peroxisomes, fluid shear stress and atherosclerosis. PPI network analysis indicated five ferroptosis-related hub genes, namely, HRAS, TXNRD1, NQO1, PSAT1, and SQSTM1. The experimental validation indicated that the expression levels of HRAS, TXNRD1, NQO1, and SQSTM1 were lower, while the expression level of PSAT1 was higher in ACLF model rats than in healthy rats. Our findings reveal that PSAT1, TXNRD1, HRAS, SQSTM1 and NQO1 may affect the development of ACLF by regulating ferroptotic events. These results provide a valid reference for potential mechanisms and identification in ACLF.
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