We are analyzing https://link.springer.com/article/10.1186/gb-2009-10-10-r115.

Title:
Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts | Genome Biology
Description:
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
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Keywords {🔍}

transcripts, pubmed, genes, hybrid, selection, sequence, sequencing, article, targeted, fusion, rnaseq, google, scholar, cas, data, cdna, reads, cancer, additional, genome, coverage, genomic, gene, dna, baits, table, file, central, library, sequences, transcript, method, figure, usa, methods, exon, transcriptome, illumina, target, bases, human, rna, pcr, fusions, information, variants, detected, refseq, nupxkr, nat,

Topics {✒️}

large-scale tumor-profiling studies article download pdf acute myeloid leukemia snhg3-rcc1-picalm fusion transcripts article number r115 dana-farber cancer institute allele-specific gene expression targeted rna-seq produces targeted rna-seq makes paired-end transcriptome sequencing full size image nuclear hormone-receptor genes targeted rna-seq experiments double-stranded dna library suitably high-throughput approaches expression-level information leading double-stranded cdna prepared targeted rna-seq method reverse gene-specific primers sequencing rt-pcr products hybridization-based capture approaches filter-based hybridization capture curated protein-coding transcripts fusion-transcript cdna fragments microarray-based genomic selection genomic copy-number alterations nup214-xkr3 fusions maintains snhg3-rcc1-picalm entire protein-coding region rna input material privacy choices/manage cookies authors’ original file expression-profiling results additional data files rna baits derived nup214-xkr3 fusion transcripts targeted rna-seq logical intragenic combinations health grant dp2od002750 nup214-xkr3 reads derive provide enhanced evidence biomed central expression profiling methods low-abundance transcripts observed termed rna-seq conventional rna-seq eliminate false positives futreal pa provide onefold coverage full access

Schema {🗺️}

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         headline:Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts
         description:Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
         datePublished:2009-10-16T00:00:00Z
         dateModified:2009-10-16T00:00:00Z
         pageStart:1
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            Chronic Myeloid Leukemia
            Additional Data File
            Sequence Coverage
            Fusion Transcript
            Animal Genetics and Genomics
            Human Genetics
            Plant Genetics and Genomics
            Microbial Genetics and Genomics
            Bioinformatics
            Evolutionary Biology
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      headline:Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts
      description:Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
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      dateModified:2009-10-16T00:00:00Z
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         cDNA Library
         Chronic Myeloid Leukemia
         Additional Data File
         Sequence Coverage
         Fusion Transcript
         Animal Genetics and Genomics
         Human Genetics
         Plant Genetics and Genomics
         Microbial Genetics and Genomics
         Bioinformatics
         Evolutionary Biology
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               type:PostalAddress
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               type:PostalAddress
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            address:
               name:Cancer Program, Broad Institute of MIT and Harvard, Cambridge, USA
               type:PostalAddress
            type:Organization
            name:Harvard Medical School
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      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Cancer Program, Broad Institute of MIT and Harvard, Cambridge, USA
      name:Department of Medical Oncology and Center for Cancer Genome Discovery, Dana-Farber Cancer Institute, Harvard Medical School, Boston, USA
      name:Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, Cambridge, USA

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