We are analyzing https://link.springer.com/article/10.1007/s00726-017-2436-z.

Title:
GPNA inhibits the sodium-independent transport system l for neutral amino acids | Amino Acids
Description:
l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
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Keywords {🔍}

pubmed, article, google, scholar, amino, cas, glutamine, cancer, transporter, acid, central, acids, cell, gpna, parma, content, transport, asct, lat, system, transporters, human, cells, access, chiu, mtorc, privacy, essential, cookies, research, leucine, open, growth, author, university, data, information, publish, search, inhibits, neutral, bianchi, ovidio, bussolati, uptake, targeting, ltype, slca, biol, wang,

Topics {✒️}

month download article/chapter sodium-independent transport system γ-glutamyltransferase enzyme activity nγ-aryl glutamine analogues mycn-amplified neuroblastoma cells lat1-4f2hc leu transporter amino acid transporter glutamine transporter asct2 related subjects neutral amino acids full article pdf ovidio bussolati bidirectional transport privacy choices/manage cookies valproic acid induces human oligodendroglioma cells essential amino acids serum/glutamine starvation sodium-independent influx article chiu l-glutamine 3t3-l1 cells check access instant access author correspondence decreased cell content glutamine uptake european economic area preliminary biological evaluation triple-negative basal amino acids 43 amino acids 49 article log tumour growth accepting optional cookies cancer cells reveals leucine induces phosphorylation article cite conditions privacy policy main content log research fellowship journal finder publish promotes mtorc1 activation glutamine depletion asct2 silencing asct2 activation cell lines heavy chain mtorc1 pathway

Questions {❓}

Fuchs BC, Bode BP (2005) Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?

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         headline:GPNA inhibits the sodium-independent transport system l for neutral amino acids
         description: l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
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      headline:GPNA inhibits the sodium-independent transport system l for neutral amino acids
      description: l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
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         System l
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         Life Sciences
         Proteomics
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