
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Bidirectional Transport of Amino Acids Regulates mTOR and Autophagy - PMC
Description:
Amino acids are required for activation of the mammalian target of rapamycin (mTOR) kinase which regulates protein translation, cell growth, and autophagy. Cell surface transporters that allow amino acids to enter the cell and signal to mTOR are ...
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Keywords {🔍}
lglutamine, cells, doi, slca, cell, eaa, figure, amino, pubmed, google, scholar, mtorc, acid, activation, mtor, growth, medium, starved, autophagy, min, acids, treated, phosphorylation, uptake, hela, presence, data, labeled, signaling, pmc, lleucine, required, protein, cellular, biol, regulates, article, translation, slcaslca, complex, size, tor, regulate, tsc, efflux, dmem, free, levels, figures, transport,
Topics {✒️}
gamma-l-glutamyl-p-nitroanilide hydrochloride l-γ-glutamyl-p-nitroanilide lc/ms/ms ion chromatograms l-glutamine-regulated l-leucine uptake l-glutamine-free dmem results sds-page mobility shift branched-chain amino acids l-glutamine-dependent mtor signaling tandem fluorescent-tagged lc3 lc/ms/ms measurement supplemental experimental procedures destabilizing au-rich elements pmc beta search regulate d-phenylalanine-sensitive phosphorylation l-glutamine-free medium ngamma-aryl glutamine analogues akt ser/thr kinase sharply dose-dependent suggesting suppresses s6k-s6 phosphorylation d-phenylalanine specifically inhibits gfp-slc1a5 allele insensitive acetonitrile/water solvent mixture labeled l-glutamic acid mcherry-egfp-lc3a distribution na+-dependent transporter d-phenylalanine sensitive activity express mcherry-egfp-lc3 regulating mtor-s6k1 activity removing extracellular l-glutamine l-leucine uptake stops l-leucine takes place l-glutamine-preloaded cells l-glutamine exerts control regulating l-leucine uptake l-glutamine sensitizes s6k1 ribosomal protein s6 figures nihms508562-supplement-document_s1__supplemental_experimental_procedures_and_six_figures l-glutamine preincubation experiments sustain mtorc1-s6k1 signals l-glutamine-regulated activation bidirectional transporter slc7a5/slc3a2 rapamycin-sensitive mtor complex rate-limiting sensitizing factor labeled l-glutamine uptake eif4e-cap complex assay rnai-insensitive slc1a5 cdna modulate slc7a5/slc3a2 function effluxed labeled l-glutamine serum-free dmem serum free dmem
Questions {❓}
- Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?
- Why does L-glutamine have this critical role?
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