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We are analyzing https://link.springer.com/article/10.1007/s00401-014-1338-3.

Title:
A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas | Acta Neuropathologica
Description:
Pediatric glioblastomas (GBM) are highly aggressive and lethal tumors. Recent sequencing studies have shown that ~30 % of pediatric GBM and ~80 % of diffuse intrinsic pontine gliomas show K27M mutations in the H3F3A gene, a variant encoding histone H3.3. H3F3A K27M mutations lead to global reduction in H3K27me3. Our goal was to develop biomarkers for the histopathologic detection of these tumors. Therefore, we evaluated the utility of measuring H3K27me3 global reduction as a histopathologic and prognostic biomarker and tested an antibody directed specifically against the H3.3 K27M mutation in 290 samples. The study cohort included 203 pediatric (including 38 pediatric high-grade astrocytomas) and 38 adult brain tumors of various subtypes and grades and 49 non-neoplastic reactive brain tissues. Detection of H3.3 K27M by immunohistochemistry showed 100 % sensitivity and specificity and was superior to global reduction in H3K27me3 as a biomarker in diagnosing H3F3A K27M mutations. Moreover, cases that stained positive for H3.3 K27M showed a significantly poor prognosis compared to corresponding negative tumors. These results suggest that immunohistochemical detection of H3.3 K27M is a sensitive and specific surrogate for the H3F3A K27M mutation and defines a prognostically poor subset of pediatric GBM.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {šŸ“š}

  • Education
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Content Management System {šŸ“}

What CMS is link.springer.com built with?

Custom-built

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Traffic Estimate {šŸ“ˆ}

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {šŸ”}

tumors, hkme, pediatric, hfa, brain, mutations, article, pubmed, mutant, google, scholar, histone, grade, mutation, cas, tumor, images, cases, usa, representative, astrocytomas, full, reduction, highgrade, fig, adult, staining, table, gliomas, global, showed, gbm, sequencing, analysis, glioblastomas, diffuse, samples, tissues, inset, including, specific, antibody, nonneoplastic, positive, negative, tissue, supplementary, data, astrocytoma, cohort,

Topics {āœ’ļø}

article download pdf paraffin-embedded formalin-fixed pediatric high-grade gliomas diffuse high-grade astrocytomas cns embryonal tumors pediatric high-grade astrocytomas brainstem high-grade glioma michelle madden felicella central nervous system pediatric high-grade gbm kaplan–meier survival analyses rabbit polyclonal anti-h3k27me3 multivariate survival analysis h3f3a k27m mutant facilitate sanger sequencing malignant rhabdoid h3f3a k27m mutation preferable diagnostic biomarker article venneti privacy choices/manage cookies mskcc cytology core histone mutational status isocitrate dehydrogenase mutations da diffuse astrocytoma k27m mutant protein mutant k27m inhibits high-grade astrocytomas evaluating h3f3a k27m randomly chosen areas recent sequencing analyses h3f3a g34r mutation automated analysis program histone mutant h3 pediatric brain tumors histone methylation wild-type tumors wild-type tumors [3 pediatric glioblastoma multiforme h3f3a mutations contribute common solid malignancy h3f3a coding exons h3f3a k27m mutations h3f3a k27m compared neoplastic brain tissues show nuclear staining rabbit polyclonal antibody adult brain tumors multivariate analysis recent sequencing studies choroid plexus papilloma

Questions {ā“}

  • 3 G34R mutations in pediatric primitive neuroectodermal tumors of central nervous system (CNS-PNET) and pediatric glioblastomas: possible diagnostic and therapeutic implications?

Schema {šŸ—ŗļø}

WebPage:
      mainEntity:
         headline:A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas
         description:Pediatric glioblastomas (GBM) are highly aggressive and lethal tumors. Recent sequencing studies have shown that ~30Ā % of pediatric GBM and ~80Ā % of diffuse intrinsic pontine gliomas show K27M mutations in the H3F3A gene, a variant encoding histone H3.3. H3F3A K27M mutations lead to global reduction in H3K27me3. Our goal was to develop biomarkers for the histopathologic detection of these tumors. Therefore, we evaluated the utility of measuring H3K27me3 global reduction as a histopathologic and prognostic biomarker and tested an antibody directed specifically against the H3.3 K27M mutation in 290 samples. The study cohort included 203 pediatric (including 38 pediatric high-grade astrocytomas) and 38 adult brain tumors of various subtypes and grades and 49 non-neoplastic reactive brain tissues. Detection of H3.3 K27M by immunohistochemistry showed 100Ā % sensitivity and specificity and was superior to global reduction in H3K27me3 as a biomarker in diagnosing H3F3A K27M mutations. Moreover, cases that stained positive for H3.3 K27M showed a significantly poor prognosis compared to corresponding negative tumors. These results suggest that immunohistochemical detection of H3.3 K27M is a sensitive and specific surrogate for the H3F3A K27M mutation and defines a prognostically poor subset of pediatric GBM.
         datePublished:2014-09-09T00:00:00Z
         dateModified:2014-09-09T00:00:00Z
         pageStart:743
         pageEnd:753
         sameAs:https://doi.org/10.1007/s00401-014-1338-3
         keywords:
            Pediatric glioblastoma
             H3F3A mutation
            K27M mutation
            H3K27me3
            Diagnostic biomarker
            Prognosis
            Methylation
            Epigenetics
            Pathology
            Neurosciences
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                     name:University of Pennsylvania
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               name:Arie Perry
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                        name:Department of Pathology, University of California, San Francisco, USA
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               name:Peter W. Lewis
               affiliation:
                     name:University of Wisconsin-Madison
                     address:
                        name:Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, USA
                        type:PostalAddress
                     type:Organization
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               name:Craig B. Thompson
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                     name:Memorial Sloan Kettering Cancer Center (MSKCC)
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                        name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
                        type:PostalAddress
                     type:Organization
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               name:Alexander R. Judkins
               affiliation:
                     name:Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California
                     address:
                        name:Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California, Los Angeles, USA
                        type:PostalAddress
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ScholarlyArticle:
      headline:A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas
      description:Pediatric glioblastomas (GBM) are highly aggressive and lethal tumors. Recent sequencing studies have shown that ~30Ā % of pediatric GBM and ~80Ā % of diffuse intrinsic pontine gliomas show K27M mutations in the H3F3A gene, a variant encoding histone H3.3. H3F3A K27M mutations lead to global reduction in H3K27me3. Our goal was to develop biomarkers for the histopathologic detection of these tumors. Therefore, we evaluated the utility of measuring H3K27me3 global reduction as a histopathologic and prognostic biomarker and tested an antibody directed specifically against the H3.3 K27M mutation in 290 samples. The study cohort included 203 pediatric (including 38 pediatric high-grade astrocytomas) and 38 adult brain tumors of various subtypes and grades and 49 non-neoplastic reactive brain tissues. Detection of H3.3 K27M by immunohistochemistry showed 100Ā % sensitivity and specificity and was superior to global reduction in H3K27me3 as a biomarker in diagnosing H3F3A K27M mutations. Moreover, cases that stained positive for H3.3 K27M showed a significantly poor prognosis compared to corresponding negative tumors. These results suggest that immunohistochemical detection of H3.3 K27M is a sensitive and specific surrogate for the H3F3A K27M mutation and defines a prognostically poor subset of pediatric GBM.
      datePublished:2014-09-09T00:00:00Z
      dateModified:2014-09-09T00:00:00Z
      pageStart:743
      pageEnd:753
      sameAs:https://doi.org/10.1007/s00401-014-1338-3
      keywords:
         Pediatric glioblastoma
          H3F3A mutation
         K27M mutation
         H3K27me3
         Diagnostic biomarker
         Prognosis
         Methylation
         Epigenetics
         Pathology
         Neurosciences
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         name:Acta Neuropathologica
         issn:
            1432-0533
            0001-6322
         volumeNumber:128
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            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Sriram Venneti
            affiliation:
                  name:Memorial Sloan Kettering Cancer Center (MSKCC)
                  address:
                     name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Mariarita Santi
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michelle Madden Felicella
            affiliation:
                  name:Henry Ford Health System
                  address:
                     name:Department of Pathology, Henry Ford Health System, Detroit, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Dmitry Yarilin
            affiliation:
                  name:Molecular Cytology Core Facility, MSKCC
                  address:
                     name:Molecular Cytology Core Facility, MSKCC, New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Joanna J. Phillips
            affiliation:
                  name:University of California
                  address:
                     name:Department of Pathology, University of California, San Francisco, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Lisa M. Sullivan
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Daniel Martinez
            affiliation:
                  name:University of Pennsylvania
                  address:
                     name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Arie Perry
            affiliation:
                  name:University of California
                  address:
                     name:Department of Pathology, University of California, San Francisco, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Peter W. Lewis
            affiliation:
                  name:University of Wisconsin-Madison
                  address:
                     name:Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Craig B. Thompson
            affiliation:
                  name:Memorial Sloan Kettering Cancer Center (MSKCC)
                  address:
                     name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Alexander R. Judkins
            affiliation:
                  name:Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California
                  address:
                     name:Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California, Los Angeles, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
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         name:Molecular Cytology Core Facility, MSKCC, New York, USA
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         name:Department of Pathology, University of California, San Francisco, USA
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      address:
         name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
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      address:
         name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
         type:PostalAddress
      name:University of California
      address:
         name:Department of Pathology, University of California, San Francisco, USA
         type:PostalAddress
      name:University of Wisconsin-Madison
      address:
         name:Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, USA
         type:PostalAddress
      name:Memorial Sloan Kettering Cancer Center (MSKCC)
      address:
         name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
         type:PostalAddress
      name:Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California
      address:
         name:Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California, Los Angeles, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Sriram Venneti
      affiliation:
            name:Memorial Sloan Kettering Cancer Center (MSKCC)
            address:
               name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
               type:PostalAddress
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      email:[email protected]
      name:Mariarita Santi
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
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      name:Michelle Madden Felicella
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            name:Henry Ford Health System
            address:
               name:Department of Pathology, Henry Ford Health System, Detroit, USA
               type:PostalAddress
            type:Organization
      name:Dmitry Yarilin
      affiliation:
            name:Molecular Cytology Core Facility, MSKCC
            address:
               name:Molecular Cytology Core Facility, MSKCC, New York, USA
               type:PostalAddress
            type:Organization
      name:Joanna J. Phillips
      affiliation:
            name:University of California
            address:
               name:Department of Pathology, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
      name:Lisa M. Sullivan
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Daniel Martinez
      affiliation:
            name:University of Pennsylvania
            address:
               name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Arie Perry
      affiliation:
            name:University of California
            address:
               name:Department of Pathology, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
      name:Peter W. Lewis
      affiliation:
            name:University of Wisconsin-Madison
            address:
               name:Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, USA
               type:PostalAddress
            type:Organization
      name:Craig B. Thompson
      affiliation:
            name:Memorial Sloan Kettering Cancer Center (MSKCC)
            address:
               name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
               type:PostalAddress
            type:Organization
      name:Alexander R. Judkins
      affiliation:
            name:Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California
            address:
               name:Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California, Los Angeles, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
      name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
      name:Department of Pathology, Henry Ford Health System, Detroit, USA
      name:Molecular Cytology Core Facility, MSKCC, New York, USA
      name:Department of Pathology, University of California, San Francisco, USA
      name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
      name:Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, USA
      name:Department of Pathology, University of California, San Francisco, USA
      name:Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, USA
      name:Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA
      name:Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, Keck School of Medicine University of Southern California, Los Angeles, USA

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