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Title:
A Transgenic Core Facility’s Experience in Genome Editing Revolution | SpringerLink
Description:
The use of animal models, particularly rodents, has been immensely important to nearly all aspects of biomedical research, from basic science exploration to translational discoveries into clinical applications. The transgenic core facility that provides animal model...
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Keywords {🔍}

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Topics {✒️}

crispr/cas-mediated genome engineering high-fidelity crispr-cas9 nucleases crispr-cas9-mediated gene inactivation crispr-cas9 genome-editing systems month download article/chapter cas9 rna-guided endonuclease rna-programmed genome editing rna-guided cas9 nucleases optimized crispr/cas system integrase-defective lentiviral vectors cas9 rna-guided endonucleases flexible guide-rna design crispr-cas9-mediated mutagenesis �yueh-chiang hu ph yueh-chiang hu ph genome-wide cas9/grna crispr/cas systems crispr-cas systems single guided rna crispr-cas system single-guide rnas genome editing revolution targeted genome engineering rna-guided endonucleases rna-guided platform privacy choices/manage cookies genome editing technologies functional guide rnas optimized sgrna parameters editing dna methylation designing crispr sgrna multiplex genome engineering highly active sgrnas detectable genome-wide device instant download direct genomic editing chapter precision medicine genome engineering celvie cas9 protein transfection hospital medical center crispr-cas9 targeting crispr/cas9 system crispr-cas9 system inactive crispr-cas9 cas9-modified mice crispr/cas cell stem cell generating knockout mice crispr/cas9 technology optimized sgrna design

Schema {🗺️}

ScholarlyArticle:
      headline:A Transgenic Core Facility’s Experience in Genome Editing Revolution
      pageEnd:90
      pageStart:75
      image:https://media.springernature.com/w153/springer-static/cover/book/978-3-319-63904-8.jpg
      genre:
         Biomedical and Life Sciences
         Biomedical and Life Sciences (R0)
      isPartOf:
         name:Precision Medicine, CRISPR, and Genome Engineering
         isbn:
            978-3-319-63904-8
            978-3-319-63903-1
         type:Book
      publisher:
         name:Springer International Publishing
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Celvie L. Yuan
            affiliation:
                  name:Cincinnati Children’s Hospital Medical Center
                  address:
                     name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yueh-Chiang Hu
            affiliation:
                  name:Cincinnati Children’s Hospital Medical Center
                  address:
                     name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:CRISPR/Cas9, Microinjection, Transgenic facility, Animal model, Genome editing, sgRNA
      description:The use of animal models, particularly rodents, has been immensely important to nearly all aspects of biomedical research, from basic science exploration to translational discoveries into clinical applications. The transgenic core facility that provides animal model production, preservation, and recovery services has been fundamental to the success of research efforts using animals. Recent advances in genome editing technologies, especially the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) enzyme system, have transformed the tedious animal model production into a simple and effective procedure. We, as a transgenic core facility established in 1993, adopted the CRISPR/Cas9 technology in early 2014 and have experienced the dramatic shift in the practice of animal model production, from the conventional embryonic stem cell approach to the direct genomic editing in rodent embryos. In this chapter, we describe the lessons that we learned from more than 200 genome editing projects performed in this core facility within the past 3 years. We also provide the practical guidelines for efficient generation of animal models using this technology and the insights into where new technologies lead us.
      datePublished:2017
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Book:
      name:Precision Medicine, CRISPR, and Genome Engineering
      isbn:
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         978-3-319-63903-1
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      name:Springer International Publishing
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      name:Cincinnati Children’s Hospital Medical Center
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      name:Celvie L. Yuan
      affiliation:
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            address:
               name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
               type:PostalAddress
            type:Organization
      name:Yueh-Chiang Hu
      affiliation:
            name:Cincinnati Children’s Hospital Medical Center
            address:
               name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
      name:Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, USA
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