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Keywords {🔍}

cas, protein, dna, doi, pubmed, google, scholar, injection, pmc, mouse, cells, genomic, gene, article, mice, fig, free, cassette, genome, efficiency, lgr, mrna, embryos, rat, deletion, crisprcas, locus, sequence, knockin, target, offtarget, generation, cell, pups, stem, gfp, hdr, nfatc, strategy, knockout, large, targeting, sgrna, data, rats, mutations, editing, system, mutation, insertion,

Topics {✒️}

watson-crick base-paring rules reverse-oriented sa-gfp-pa cassette 5 mg/ml x-gal dissolved rosa26-lsl-lacz reporter strain cre-loxp-mediated dna inversion35 crispr-cas rna-guided system crispr/cas-mediated genome editing crispr/cas-mediated genome engineering crispr/cas-based methods reverse oriented sa-gfp-pa large-scale genome-modified mice pmc beta search wild-type cellular environment identically-oriented loxp pairs crispr/cas nuclease pairs monitoring cre-mediated recombination rosa26-lsl-lacz mice rna-guided crispr cas9 cationic lipid-mediated delivery primer pair rt1&rt3 primer pair rt1&rt2 heterospecific loxp twin-sites site-specific genomic engineering 10–50 ng/ul dna donor rna-guided cas9 nucleases l-sa-gfp-pa gene-trapped mouse models 25 ng/μl cas9 mrna cas9/sgrna plasmid dna wild-type allele x-gal staining loxp-inserted conditional alleles site-specific inserted pups cas9 protein/sgrna system talen-mediated gene targeting large genomic fragment 7 kb ires-creert2 cassette nfatc1-ires-creert2 knock cas9 mrna slightly increased developing therapeutic strategies1 human protein-coding genes wild-type rat lgr5-gfp fusion mrna rna-guided endonucleases crispr/cas system crispr-cas system simultaneously labeling cells license information pmcid f1 nfatc1-creert2 mice rosa26-lacz+/− mouse

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Emails and Hosting {✉️}

Mail Servers:

• nihcesxway.hub.nih.gov
• nihcesxway2.hub.nih.gov
• nihcesxway3.hub.nih.gov
• nihcesxway4.hub.nih.gov
• nihcesxway5.hub.nih.gov

Name Servers:

• dns1-ncbi.ncbi.nlm.nih.gov
• dns2-ncbi.ncbi.nlm.nih.gov
• lhcns1.nlm.nih.gov
• lhcns2.nlm.nih.gov

CDN Services {📦}

• Ncbi