
NCBI . NLM . NIH . GOV {
}
Title[redir]:
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering - PMC
Description:
Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient ...
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Keywords {🔍}
tet, mice, doi, targeting, cas, mutations, cells, gene, alleles, genes, figure, pubmed, google, scholar, genome, sgrnas, pmc, mrna, targeted, dna, mouse, mutant, article, analysis, embryos, cell, editing, sgrna, pcr, rflp, free, table, carrying, multiple, crisprcas, system, sequence, ngμl, site, target, injected, clones, generation, high, number, assay, generated, potential, multiplexed, zygotes,
Topics {✒️}
crispr/cas-mediated double-gene targeting crispr/cas-mediated single-gene targeting crispr/cas-mediated genome editing crispr/cas-mediated gene editing crispr/cas-mediated gene targeting cas9/rna-mediated gene targeting mammalian-codon-optimized cas9 supplementary materials included nih grants r37-hd045022 plasmid expressing pgk-puror hdr-mediated precise mutations crispr/cas-mediated targeting synthetic single-guide rna avoids time-consuming intercrossing tet1/tet2 double-mutant mice pmc beta search cas9 rna-guided endonuclease make double-mutant mice single-stranded dna oligos rnase-free water single-gene modification prompted rna-guided cas9 nuclease double-gene mutant mice double-mutant mice produced nhej-mediated gene mutations nihms539744-supplement-supplement_1 talen-mediated gene targeting experimental procedures procedures t7-cas9 pcr product efficient gene-targeting technology high live-birth rate single-gene mutant mice conventional gene-targeting methods high-frequency genome editing triple-targeted es cells references bogdanove aj t7-sgrna pcr product double-strand breaks nhej-based gene disruption cas9 expressing plasmid traditional gene-targeting methods high-fidelity homologous recombination mediated genome editing form ribonucleoprotein complexes precise genome editing tet2 single-targeted mice crispr/cas systems crispr-cas systems tet1 single-targeted mice rna-guided editing
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