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We began analyzing https://stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1012-0, but it redirected us to https://stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1012-0. The analysis below is for the second page.

Title[redir]:
Human tissue-specific MSCs demonstrate differential mitochondria transfer abilities that may determine their regenerative abilities | Stem Cell Research & Therapy | Full Text
Description:
Background Recent studies have demonstrated mesenchymal stem cells (MSCs) as effective mitochondrial donors with therapeutic success in multiple experimental models of human disease. MSCs obtained from different tissue sources such as bone marrow (BM), adipose (AD), dental pulp (DP), and Wharton’s jelly (WJ) are routinely used in clinical trials with no known study of their mitochondrial donor capacity. Here, we show for the first time that MSCs derived from different tissue sources have different mitochondrial donor properties and that this is correlated with their intrinsic respiratory states. Methods MitoTracker®-labeled MSCs were co-cultured with Cell Trace–labeled U87-MG cells or rat cardiomyocytes. Mitochondrial transfer abilities of MSCs were assessed by using flow cytometry analysis and fluorescence imaging. Mitochondrial reactive oxygen species (mtROS) levels were analyzed by using MitoSOX red–based staining, and mitochondrial respiration parameters were analyzed by using a Seahorse XF Analyzer. Results AD-MSCs and BM-MSCs displayed higher mitochondrial transfer than DP-MSCs and WJ-MSCs. Counterintuitively, DP-MSCs and WJ-MSCs were more effective in suppressing mtROS levels in stressed recipient cells than AD-MSCs or BM-MSCs. Interestingly, the oxygen consumption rates and intrinsic mitochondrial respiration parameters like ATP levels, basal and maximal respiration, and mitochondrial DNA copy number in donor MSCs showed a highly significant inverse correlation with their mitochondrial donation. Conclusions We find that there are intrinsic differences in the mitochondrial respiration, donation capacity, and therapeutic efficacy among MSCs of different tissue origin. MSCs with high mitochondrial respiration capacities are associated with lower mitochondrial transfer but more effective suppression of mtROS in stressed recipient cells. This is most compatible with a model where recipient cells optimally regulate mitochondrial transfer such that they take more mitochondria from MSCs with lower mitochondrial function. Furthermore, it appears to be advantageous to use MSCs such as DP-MSCs or WJ-MSCs with higher mitochondrial respiratory abilities that achieved better therapeutic effect with lower mitochondrial transfer in our study. This opens up a new direction in stem cell therapeutics.

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mitochondrial, cells, mscs, transfer, cell, article, stem, mitochondria, tissuespecific, google, scholar, cas, data, mesenchymal, bmmscs, fig, mtros, levels, admscs, dpmscs, cardiomyocytes, wjmscs, umg, human, number, flow, respiratory, parameters, analysis, differential, tissue, study, respiration, recipient, shown, rescue, donor, atp, mtdna, observed, cytometry, higher, abilities, oxygen, potential, reduction, copy, oxidative, sources, capacity,

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author information authors springer nature bone-marrow-derived stromal cells results mitochondrial transfer bmc cell biol state privacy rights cryopreserved tissue-specific mscs nuclear beta-actin gene actin-dependent mitochondrial internalization human tissue-specific mscs methods revival intercellular organelle transport original author neonatal tissue-derived msc total protein content representative tissue-specific mscs structure-mediated mitochondrial transfer tissue-specific mscs differed results ad-mscs abbreviations ad rescue anthracycline-induced cardiomyopathy enzyme-linked immunosorbent assay stem cell research inter-cellular mitochondrial transfer mesenchymal stem cells percentage double-positive cells activating anti-oxidant mechanisms vitro ischemia-reperfusion model tissue-specific donor mscs recipient u87-mg cells donor tissue-specific groups stressed u87-mg cells mesenchymal stromal cells change rights tissue-specific mscs obtained ventilator-induced lung injury live cell microscopy abbreviations mitotracker® green–labeled mitochondria cell stem cell conclusions high intrinsic miro1 tissue origins demonstrate mitosox red–stained untreated antimycin-treated u87-mg mitosox red–based staining stem cell facility privacy choices/manage cookies differential mitochondrial transfer

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