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We are analyzing https://link.springer.com/article/10.1186/1471-2121-11-29.

Title:
Mesenchymal stem cells rescue cardiomyoblasts from cell death in an in vitro ischemia model via direct cell-to-cell connections | BMC Molecular and Cell Biology
Description:
Background Bone marrow derived mesenchymal stem cells (MSCs) are promising candidates for cell based therapies in myocardial infarction. However, the exact underlying cellular mechanisms are still not fully understood. Our aim was to explore the possible role of direct cell-to-cell interaction between ischemic H9c2 cardiomyoblasts and normal MSCs. Using an in vitro ischemia model of 150 minutes of oxygen glucose deprivation we investigated cell viability and cell interactions with confocal microscopy and flow cytometry. Results Our model revealed that adding normal MSCs to the ischemic cell population significantly decreased the ratio of dead H9c2 cells (H9c2 only: 0.85 ± 0.086 vs. H9c2+MSCs: 0.16 ± 0.035). This effect was dependent on direct cell-to-cell contact since co-cultivation with MSCs cultured in cell inserts did not exert the same beneficial effect (ratio of dead H9c2 cells: 0.90 ± 0.055). Confocal microscopy revealed that cardiomyoblasts and MSCs frequently formed 200-500 nm wide intercellular connections and cell fusion rarely occurred between these cells. Conclusion Based on these results we hypothesize that mesenchymal stem cells may reduce the number of dead cardiomyoblasts after ischemic damage via direct cell-to-cell interactions and intercellular tubular connections may play an important role in these processes.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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Link.springer.com utilizes APACHE LENYA.

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🌠 Phenomenal Traffic: 5M - 10M visitors per month

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Keywords {🔍}

cells, cell, stem, mscs, article, ogd, google, scholar, pubmed, cardiomyoblasts, figure, mesenchymal, fusion, hours, cas, celltocell, vitro, cardiomyocytes, culture, time, flow, dead, bone, red, double, analysis, direct, connections, microscopy, labeled, cytometry, file, model, number, marrow, damaged, ischemia, effect, heart, added, authors, res, ischemic, minutes, confocal, intercellular, cardiac, tissue, found, paracrine,

Topics {✒️}

levente kiss & zsombor lacza bone-marrow-derived cells oxygen glucose deprivation time-lapse video microscopy article download pdf rescue post-ischemic cardiomyoblasts mesenchymal stem cells hungarian science mesenchymal stem cell bone marrow cells short-lived tubular connections rescue factor time lapse pictures rescuing post-ischemic cardiomyoblasts related subjects modifying ventricular remodeling post-ischemic cardiomyoblasts interact dio-labeled h9c2 cells cardiovascular revascularization medicine stem cell transplantation haematopoietic stem cells labeled stem cell full size image privacy choices/manage cookies stem cell grafting diseases glucose-free dmem brain research protocols stem cells failed clinical experimental research cardiac cell lineages bone marrow complete rightward shift rescue aerobic respiration cardiac myocytes leading garcia-verdugo jm low glucose dmem flow cytometry files authors’ original file preserve heart tissue ischemically damaged cells full access partial cell fusion post-ischemic cardiomyoblasts heart tissue repair healthy cells contacted side scatter plot constantly changing web postinfarcted rat myocardium data represent

Questions {❓}

Kaplan LD: The analysis of articular cartilage after thermal exposure: "Is red really dead?
Koyanagi M, Brandes RP, Haendeler J, Zeiher AM, Dimmeler S: Cell-to-Cell Connection of Endothelial Progenitor Cells With Cardiac Myocytes by Nanotubes: A Novel Mechanism for Cell Fate Changes?

Schema {🗺️}

WebPage:
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