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We began analyzing https://biosignaling.biomedcentral.com/articles/10.1186/s12964-022-00979-0, but it redirected us to https://biosignaling.biomedcentral.com/articles/10.1186/s12964-022-00979-0. The analysis below is for the second page.

Title[redir]:
The ERα-NRF2 signalling axis promotes bicalutamide resistance in prostate cancer | Cell Communication and Signaling | Full Text
Description:
Background Bicalutamide is a nonsteroidal antiandrogen widely used as a first-line clinical treatment for advanced prostate cancer (PCa). Although patients initially show effective responses to bicalutamide treatment, resistance to bicalutamide frequently occurs and leads to the development of castration-resistant PCa (CRPC). This research investigated the roles of the oestrogen receptor α (ERα)-nuclear factor E2-related factor 2 (NRF2) signalling pathway in bicalutamide resistance in PCa cells. Methods We performed bioinformatic analysis and immunohistochemical staining on normal and cancerous prostate tissue to evaluate ERα and NRF2 expression and their correlation. Gene expression and localization in PCa cell lines were further investigated using real-time reverse transcription PCR/Western blotting and immunofluorescence staining. We treated PCa cells with the ER inhibitor tamoxifen and performed luciferase reporter assays and chromatin immunoprecipitation (ChIP) assays to understand ERα-dependent NRF2 expression. Overexpression and knockdown of ERα and NRF2 were used to explore the potential role of the ERα-NRF2 signalling axis in bicalutamide resistance in PCa cells. Results We found that the expression of ERα and NRF2 was positively correlated and was higher in human CRPC tissues than in primary PCa tissues. Treatment with oestrogen or bicalutamide increased the expression of ERα and NRF2 as well as NRF2 target genes in PCa cell lines. These effects were blocked by pretreatment with tamoxifen. ChIP assays demonstrated that ERα directly binds to the oestrogen response element (ERE) in the NRF2 promoter. This binding led to increased transcriptional activity of NRF2 in a luciferase reporter assay. Activation of the ERα-NRF2 signalling axis increased the expression of bicalutamide resistance-related genes. Inhibition of this signalling axis by knockdown of ERα or NRF2 downregulated the expression of bicalutamide resistance-related genes and inhibited the proliferation and migration of PCa cells. Conclusions We demonstrated the transcriptional interaction between ERα and NRF2 in CRPC tissues and cell lines by showing the direct binding of ERα to the ERE in the NRF2 promoter under oestrogen treatment. Activation of the ERα-NRF2 signalling axis contributes to bicalutamide resistance in PCa cells, suggesting that the ERα-NRF2 signalling axis is a potential therapeutic target for CRPC. Video Abstract

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nrf, cells, erα, expression, lncap, bicalutamide, pca, cancer, pubmed, prostate, article, google, scholar, abl, cell, resistance, treatment, fig, cas, analysis, signalling, crpc, genes, oestrogen, central, zhang, receptor, pathway, tissues, activation, additional, knockdown, file, treated, promoter, data, primary, estrogen, axis, lines, ere, erαnrf, normal, gene, results, increased, samples, performed, activity, mrna,

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verification code 22d8-bec0-6798-0502-f91e bicalutamide-induced apoptosis remains erα-mediated oestrogen-dependent manner cerebral ischemia-reperfusion injury embedded gene-set nodes bmc syst biol dual-luciferase assay kit cerebral ischaemia‒reperfusion model incurable castration-resistant pca castration-resistant prostate cancer repress nrf2-mediated transcription oxidative-induced cell damage clinically-tested nrf2-activators epithelial-mesenchymal transition phenotypes aromatase-induced oestrogen promotes nrf2-dependent detoxification enzyme estrogen receptor-dependent activation erα-nrf2 signalling axis erα–nrf2 signalling axis culture virus-infected cells small interfering rna account cell communication nrf2/sirt3/mnsod signaling nrf2-antioxidative signaling pathways androgen-independent pca cells bicalutamide resistance-related genes ros-independent nrf2 activation e2-bound erα interacts signalling axis activity cell type-specific activation bmc cancer erα-nrf2 signalling pathway privacy choices/manage cookies estrogen receptor-alpha estrogen receptor alpha pivotal transcription factor transcription factor sp1 erα-mediated downstream effect estrogen receptor beta authors scientific editing mitochondrion-derived superoxide erα-mediated nrf2 expression castration-resistant pca reactive oxygen species underwent surgical castration estrogen signaling activates estrogen receptor signaling prostatic stromal cells 0 µm pore size free radic res

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Video Abstract

Background

Prostate cancer (PCa) is the second most common cancer and the fifth leading cause of cancer-related death in men [5.57s.