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  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
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  6. Keywords
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We began analyzing https://bmcbiochem.biomedcentral.com/articles/10.1186/s12858-015-0042-9, but it redirected us to https://bmcbiochem.biomedcentral.com/articles/10.1186/s12858-015-0042-9. The analysis below is for the second page.

Title[redir]:
The androgen receptor plays a suppressive role in epithelial- mesenchymal transition of human prostate cancer stem progenitor cells | BMC Biochemistry | Full Text
Description:
Background To investigate the roles of androgen receptor (AR) in epithelial- mesenchymal transition (EMT) in human prostate cancer stem progenitor (S/P) cells isolated from LNCaP cell line. Methods The S/P cells were obtained from LNCaP cell line through florescence-activated cell sorting (FACS). AR was overexpressed in S/P cells through lentivirus. Western blot assay was used to detect the EMT markers expression, such as E Cadherin, N Cadherin, Vimentin and Snail. MTT assay, soft agar colony formation assay, sphere formation assay and migration assay were used to investigate AR’s roles in EMT of S/P cells. Cell signaling pathways associated with proliferation and apoptosis of S/P cells were detected simultaneously. And S/P cells were treated with in vitro combinatory use of LY 294002 (inhibitor of AKT signaling molecules) with γ-TT and/or 5-AZA. Results Our data showed that S/P cells from LNCaP had high EMT markers expression, more tumorigenesis and strong migration ability. And in S/P cells overexpressed with AR, the expression of EMT markers decreased. In addition, these cells had less proliferation ability, tumorigenesis ability, self-renewal and migration ability. At the same time, targeting S/P cells with AKT signaling pathway inhibitor LY29004 andγ-TT and/or 5-AZA could inhibit S/P cell’s proliferation and tumorigenesis. Conclusions Our data suggest that AR played a negative role in EMT of PCa S/P cells, by regulating AKT cell signaling pathway, which could be a new strategy to treat castration resistant prostate cancer (CRPC).

Matching Content Categories {📚}

  • Science
  • Education
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Content Management System {📝}

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Custom-built

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🚗 Small Traffic: 1k - 5k visitors per month


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Keywords {🔍}

cells, cancer, cell, prostate, emt, pubmed, article, google, scholar, signaling, expression, assay, stem, cas, lncap, markers, figure, androgen, epithelial, transition, proliferation, central, cadherin, analysis, receptor, bcl, akt, pca, medium, line, agar, pathways, tumor, days, formation, cmyc, incubated, data, role, human, min, western, showed, ability, characteristics, staining, antibodies, bmc, blot, mtt,

Topics {✒️}

springer nature author information authors c-jun-dependent transcriptional activation bmp7-induced epithelial-mesenchymal transition inhibits epithelial-mesenchymal transition abbreviations ar wnt/β-catenin signal network state privacy rights conclusion methods cell lines prostate cancer stem/progenitor sds/page gel wu nan & zhang shuhai prostate stem/progenitor cells cancer stem/progenitor cells qrt-pcr analysis results pi3k/akt/mtor pathway author correspondence pten/akt/pi3k signaling florescence-activated cell sorting single-color positive controls epithelial- mesenchymal transition epithelial-mesenchymal transition background subtraction docetaxel-resistant pca cells c-myc sirna plasmids information rights hrp-conjugated secondary antibodies proliferation include pi3k/akt egfr-mediated erk signaling stem/progenitor cells stem/progenitor cells androgen/androgen receptor privacy choices/manage cookies quantitative pcr analysis soft agar assay bmc cancer cell stem cell epithelial cell marker wnt signaling regulates sphere formation assay androgen receptor plays authors scientific editing cancer stem cell cancer stem cell maintain stem cell erk signaling pathway notch1 signaling pathway cell signaling pathways

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Social Networks {👍}(2)

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Analytics and Tracking {📊}

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Libraries {📚}

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Emails and Hosting {✉️}

Mail Servers:

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  • mx3.zoho.eu

Name Servers:

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CDN Services {📦}

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