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Title[redir]:
The role of PTEN/Akt/PI3K signaling in the maintenance and viability of prostate cancer stem-like cell populations - PMC
Description:
Characterization of the molecular pathways that are required for the viability and maintenance of self-renewing tumor-initiating cells may ultimately lead to improved therapies for cancer. In this study, we show that a CD133+/CD44+ population of ...
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Keywords {π}
cells, cell, cancer, doi, foxoa, prostate, conditions, fig, pik, grown, pcaps, google, scholar, tumors, pubmed, cdcd, pten, pathway, signaling, sphereforming, knockdown, analysis, monolayer, increase, maintenance, compared, sphere, potential, tumor, observed, showed, role, increased, expression, stemlike, data, mice, genes, control, populations, tumorigenic, progenitors, levels, human, pmc, lines, shrna, nvpbez, fold, inhibition,
Topics {βοΈ}
org/cgi/content/full/0810956106/dcsupplemental anchorage-independent serum-free media primary human low-grade facs-sorted cd133+/cd44+ pi3k/mtor/pten signaling pten/mtor/stat3 pathway high-grade prostate tumors long-term culture conditions bcr-ablβtransformed cells bcr-abl-transformed cells 10-cm low-attachment dishes pmc beta search hormone-resistant prostate cancer serum-free ebm supplemented quantitative rt-pcr confirmation gadd45-luc reporter vector demonstrated lineage-restricted tumorigenesis long-term monolayer cultures single-color positive controls examine anchorage-independent growth sphere-specific signaling pathways stably introduced foxo3a-gfp cd133+/cd44+ cell-enriched pcaps pten/pi3k network plays transplantable leukemia-initiating cells pi3k/akt signaling pathway pten/akt/pi3k signaling pten/pi3k/akt pathways pten-positive du145 cells double-positive cells compared renewing tumor-initiating cells sphere-forming ability compared cd44+/cd133+ population compared increased sphere-forming ability cd133β/cd44β du145 cells sphere-forming conditions compared pi3k inhibitor nvp-bez235 cd133β/cd44β cells compared supporting information online flag-tagged wt foxo3a serum-free conditions /pi3k/akt1/foxo3a signaling determine cd44+/cd133+ populations serum-free ebm pten-negative pc3 cells dmso-treated control cells cellular localization data mg2+-free pbs scrambled control cells tumor-initiating potential
Questions {β}
- Cancer stem-like cells in human prostate carcinoma cells DU145: The seeds of the cell line?
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