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We began analyzing https://www.nature.com/articles/nprot.2006.31, but it redirected us to https://www.nature.com/articles/nprot.2006.31. The analysis below is for the second page.

Title[redir]:
Reconstruction of human mammary tissues in a mouse model | Nature Protocols
Description:
Establishing a model system that more accurately recapitulates both normal and neoplastic breast epithelial development in rodents is central to studying human breast carcinogenesis. However, the inability of human breast epithelial cells to colonize mouse mammary fat pads is problematic. Considering that the human breast is a more fibrous tissue than is the adipose-rich stroma of the murine mammary gland, our group sought to bypass the effects of the rodent microenvironment through incorporation of human stromal fibroblasts. We have been successful in reproducibly recreating functionally normal breast tissues from reduction mammoplasty tissues, in what we term the human-in-mouse (HIM) model. Here we describe our relatively simple and inexpensive techniques for generating this orthotopic xenograft model. Whether the model is to be applied for understanding normal human breast development or tumorigenesis, investigators with minimal animal surgery skills, basic cell culture techniques and access to human breast tissue will be able to generate humanized mouse glands within 3 months. Clearing the mouse of its endogenous epithelium with subsequent stromal humanization takes 1 month. The subsequent implantation of co-mixed human epithelial cells and stromal cells occurs 2 weeks after humanization, so investigators should expect to observe the desired outgrowths 2 months afterward. As a whole, this model system has the potential to improve the understanding of crosstalk between tissue stroma and the epithelium as well as factors involved in breast stem cell biology tumor initiation and progression.

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Keywords {🔍}

breast, article, nature, mammary, human, scholar, cancer, google, pubmed, access, cas, mouse, kuperwasser, tissue, content, tissues, model, epithelial, cells, cell, research, cookies, normal, gland, privacy, charlotte, development, stromal, stem, biology, open, mice, res, data, protocols, deome, advertising, information, journal, subscribe, june, reconstruction, proia, fat, stroma, microenvironment, fibroblasts, epithelium, institution, buy,

Topics {✒️}

nature portfolio permissions reprints privacy policy breast cancer research mammary-gland-free fat-pads advertising nature social media tissue research author information authors carcinogen-induced mammary tumors tumor-stromal interactions author correspondence cancer stem cells personal data data protection permissions springerlink instant access unirradiated epithelial cells neoplastic mammary tissues mammary gland biology breast tissue microenvironment human mammary tissues ddr1 gat raunerdexter murine mammary gland rb sackler scholar human stromal fibroblasts cleared fat pad pancreatic cancer cells privacy human mammary fibroblasts mouse mammary glands reduction mammoplasty tissues development article proia human breast tissue breast tissue regeneration human breast epithelia preneoplastic nodule outgrowths explore content subscription content adipose-rich stroma european economic area institutional subscriptions read diminished gonadotropin concentration kluwer academic/plenum susan komen foundations england medical center patent application based combination therapy ian

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      headline:Reconstruction of human mammary tissues in a mouse model
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