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Title:
The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells | SpringerLink
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The mammary fat pad, when cleared of glandular tissues in female mice 3–4 weeks old, provides an ideal site for the transplantation of normal, hyperplastic, or malignant lesions of the mammary gland into syngeneic hosts. The cleared fat pad retains the...
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Keywords {๐}
mammary, google, scholar, cancer, cas, gland, pubmed, mouse, chapter, cells, young, mice, transplantation, deome, res, fat, article, research, breast, lesions, growth, faulkin, cleared, pad, hyperplastic, human, natl, inst, cardiff, privacy, cookies, content, information, publish, tissues, normal, osborn, guzman, analysis, search, biology, transplanted, serial, tissue, preview, epithelial, daniel, life, span, preneoplastic,
Topics {โ๏ธ}
inapparent nodule-transformed cells ultra-short-range effector carcinogen-treated c57b1 mice mouse mammary epithelium pubmedย google scholar casย google scholar chapterย google scholar privacy choices/manage cookies mammary fat pad mammary gland structures breast cancer research mouse mammary glands secretory epithelial cells cleared fat pads balb/cfc3h mice ductal mammary cells brown fat pads mammary gland phenotypes rat mammary glands mammary gland biology mammary gland tissues murine mammary cancer powerful biological system carcinogen-induced transformation download preview pdf mammary gland biol neoplastic mammary tissues european economic area female c3h mice journal finder publish nude athymic mouse conditions privacy policy unlimited division potential c3h/crgl mice accepting optional cookies breast cancer res acta union int transformed nodule neu/erbb-2 oncogene normal morphological growth google scholar main content log mammary gland mammary tumorigenesis chapter cite social media permissions reprints gland elements chapter methods tumor progression
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ScholarlyArticle:
headline:The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells
pageEnd:74
pageStart:67
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4615-4295-7.jpg
genre:
Biomedical and Life Sciences
Biomedical and Life Sciences (R0)
isPartOf:
name:Methods in Mammary Gland Biology and Breast Cancer Research
isbn:
978-1-4615-4295-7
978-1-4613-6927-1
type:Book
publisher:
name:Springer US
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Lawrence J. T. Young
affiliation:
name:University of California-Davis
address:
name:Center for Comparative Medicine, University of California-Davis, Davis, USA
type:PostalAddress
type:Organization
type:Person
keywords:Mammary Gland, Mouse Mammary Gland, Serial Transplantation, Human Mammary Gland, Normal Mammary Tissue
description:The mammary fat pad, when cleared of glandular tissues in female mice 3โ4 weeks old, provides an ideal site for the transplantation of normal, hyperplastic, or malignant lesions of the mammary gland into syngeneic hosts. The cleared fat pad retains the microenvironment necessary for the normal morphological growth of transplanted mammary gland elements. The biological development of mammary gland structures can be evaluated and lesions can be tested for cancer risk. Serial transplantation into cleared fat pads can provide pools of the tissue of choice for biochemical, immunological, and molecular analysis, for tissue culture, and for the perpetuation of hyperplastic tissues for future use. With the advent of transgenic and knockout mice, the clearing technique has become a powerful biological system wherein the effect of genes and their interactions on mammary neoplasia can be determined. This chapter describes the clearing and transplantation technique for mammary tissues and cells.
datePublished:2000
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affiliation:
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