NATURE . COM {}

Matching Content Categories {📚}

• Non-Profit & Charity
• Education
• Science

Content Management System {📝}

What CMS is nature.com built with?

Custom-built

No common CMS systems were detected on Nature.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of nature.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Nature.com Make Money? {💸}

Display Ads {🎯}

Direct Advertisers (10)

Reseller Advertisers (38)

How Much Does Nature.com Make? {💰}

Display Ads {🎯}

$63,100 per month
Our estimates place Nature.com's monthly online earnings from display ads at $42,042 to $115,616.

Keywords {🔍}

breast, article, nature, mammary, human, scholar, cancer, google, pubmed, access, cas, mouse, kuperwasser, tissue, content, tissues, model, epithelial, cells, cell, research, cookies, normal, gland, privacy, charlotte, development, stromal, stem, biology, open, mice, res, data, protocols, deome, advertising, information, journal, subscribe, june, reconstruction, proia, fat, stroma, microenvironment, fibroblasts, epithelium, institution, buy,

Topics {✒️}

nature portfolio permissions reprints privacy policy breast cancer research mammary-gland-free fat-pads advertising nature social media tissue research author information authors carcinogen-induced mammary tumors tumor-stromal interactions author correspondence cancer stem cells personal data data protection permissions springerlink instant access unirradiated epithelial cells neoplastic mammary tissues mammary gland biology breast tissue microenvironment human mammary tissues ddr1 gat raunerdexter murine mammary gland rb sackler scholar human stromal fibroblasts cleared fat pad pancreatic cancer cells privacy human mammary fibroblasts mouse mammary glands reduction mammoplasty tissues development article proia human breast tissue breast tissue regeneration human breast epithelia preneoplastic nodule outgrowths explore content subscription content adipose-rich stroma european economic area institutional subscriptions read diminished gonadotropin concentration kluwer academic/plenum susan komen foundations england medical center patent application based combination therapy ian

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Reconstruction of human mammary tissues in a mouse model
         description:Establishing a model system that more accurately recapitulates both normal and neoplastic breast epithelial development in rodents is central to studying human breast carcinogenesis. However, the inability of human breast epithelial cells to colonize mouse mammary fat pads is problematic. Considering that the human breast is a more fibrous tissue than is the adipose-rich stroma of the murine mammary gland, our group sought to bypass the effects of the rodent microenvironment through incorporation of human stromal fibroblasts. We have been successful in reproducibly recreating functionally normal breast tissues from reduction mammoplasty tissues, in what we term the human-in-mouse (HIM) model. Here we describe our relatively simple and inexpensive techniques for generating this orthotopic xenograft model. Whether the model is to be applied for understanding normal human breast development or tumorigenesis, investigators with minimal animal surgery skills, basic cell culture techniques and access to human breast tissue will be able to generate humanized mouse glands within 3 months. Clearing the mouse of its endogenous epithelium with subsequent stromal humanization takes 1 month. The subsequent implantation of co-mixed human epithelial cells and stromal cells occurs 2 weeks after humanization, so investigators should expect to observe the desired outgrowths 2 months afterward. As a whole, this model system has the potential to improve the understanding of crosstalk between tissue stroma and the epithelium as well as factors involved in breast stem cell biology tumor initiation and progression.
         datePublished:2006-06-27T00:00:00Z
         dateModified:2006-06-27T00:00:00Z
         pageStart:206
         pageEnd:214
         sameAs:https://doi.org/10.1038/nprot.2006.31
         keywords:
            Life Sciences
            general
            Biological Techniques
            Analytical Chemistry
            Microarrays
            Computational Biology/Bioinformatics
            Organic Chemistry
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fnprot.2006.31/MediaObjects/41596_2006_BFnprot200631_Fig1_HTML.gif
         isPartOf:
            name:Nature Protocols
            issn:
               1750-2799
               1754-2189
            volumeNumber:1
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Nature Publishing Group UK
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:David A Proia
               affiliation:
                     name:Tufts University School of Medicine
                     address:
                        name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Charlotte Kuperwasser
               affiliation:
                     name:Tufts University School of Medicine
                     address:
                        name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
                        type:PostalAddress
                     type:Organization
                     name:Tufts-New England Medical Center, Molecular Oncology Research Institute
                     address:
                        name:Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Reconstruction of human mammary tissues in a mouse model
      description:Establishing a model system that more accurately recapitulates both normal and neoplastic breast epithelial development in rodents is central to studying human breast carcinogenesis. However, the inability of human breast epithelial cells to colonize mouse mammary fat pads is problematic. Considering that the human breast is a more fibrous tissue than is the adipose-rich stroma of the murine mammary gland, our group sought to bypass the effects of the rodent microenvironment through incorporation of human stromal fibroblasts. We have been successful in reproducibly recreating functionally normal breast tissues from reduction mammoplasty tissues, in what we term the human-in-mouse (HIM) model. Here we describe our relatively simple and inexpensive techniques for generating this orthotopic xenograft model. Whether the model is to be applied for understanding normal human breast development or tumorigenesis, investigators with minimal animal surgery skills, basic cell culture techniques and access to human breast tissue will be able to generate humanized mouse glands within 3 months. Clearing the mouse of its endogenous epithelium with subsequent stromal humanization takes 1 month. The subsequent implantation of co-mixed human epithelial cells and stromal cells occurs 2 weeks after humanization, so investigators should expect to observe the desired outgrowths 2 months afterward. As a whole, this model system has the potential to improve the understanding of crosstalk between tissue stroma and the epithelium as well as factors involved in breast stem cell biology tumor initiation and progression.
      datePublished:2006-06-27T00:00:00Z
      dateModified:2006-06-27T00:00:00Z
      pageStart:206
      pageEnd:214
      sameAs:https://doi.org/10.1038/nprot.2006.31
      keywords:
         Life Sciences
         general
         Biological Techniques
         Analytical Chemistry
         Microarrays
         Computational Biology/Bioinformatics
         Organic Chemistry
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fnprot.2006.31/MediaObjects/41596_2006_BFnprot200631_Fig1_HTML.gif
      isPartOf:
         name:Nature Protocols
         issn:
            1750-2799
            1754-2189
         volumeNumber:1
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Nature Publishing Group UK
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:David A Proia
            affiliation:
                  name:Tufts University School of Medicine
                  address:
                     name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Charlotte Kuperwasser
            affiliation:
                  name:Tufts University School of Medicine
                  address:
                     name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
                     type:PostalAddress
                  type:Organization
                  name:Tufts-New England Medical Center, Molecular Oncology Research Institute
                  address:
                     name:Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Nature Protocols
      issn:
         1750-2799
         1754-2189
      volumeNumber:1
Organization:
      name:Nature Publishing Group UK
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Tufts University School of Medicine
      address:
         name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
         type:PostalAddress
      name:Tufts University School of Medicine
      address:
         name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
         type:PostalAddress
      name:Tufts-New England Medical Center, Molecular Oncology Research Institute
      address:
         name:Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:David A Proia
      affiliation:
            name:Tufts University School of Medicine
            address:
               name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
               type:PostalAddress
            type:Organization
      name:Charlotte Kuperwasser
      affiliation:
            name:Tufts University School of Medicine
            address:
               name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
               type:PostalAddress
            type:Organization
            name:Tufts-New England Medical Center, Molecular Oncology Research Institute
            address:
               name:Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
      name:Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, USA
      name:Department of Radiation Oncology, Tufts-New England Medical Center, Molecular Oncology Research Institute, Boston, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

Social Networks {👍}(1)

• https://twitter.com/NatureProtocols

External Links {🔗}(55)

• How much does https://link.springer.com/article/10.1038/nprot.2006.31?utm_source=nature&utm_medium=referral&utm_campaign=buyArticle generate monthly?
• How much does http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=13663040 rake in every month?
• What is the earnings of http://scholar.google.com/scholar_lookup?&title=Development%20of%20mammary%20tumors%20from%20hyperplastic%20alveolar%20nodules%20transplanted%20into%20gland-free%20mammary%20fat%20pads%20of%20female%20C3H%20Mice&journal=Cancer%20Res&volume=19&pages=515-525&publication_year=1959&author=DeOme%2CKB&author=Faulkin%2CLJ&author=Bern%2CH&author=Blair%2CPB?
• Discover the revenue of https://doi.org/10.1126%2Fscience.149.3684.634
• How much money does http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=14331183 make?
• Get to know what's the income of http://scholar.google.com/scholar_lookup?&title=Growth%20of%20mouse%20mammary%20glands%20in%20vivo%20after%20monolayer%20culture&journal=Science&doi=10.1126%2Fscience.149.3684.634&volume=149&pages=634-636&publication_year=1965&author=Daniel%2CCW&author=DeOme%2CKB
• How much revenue does https://doi.org/10.1016%2F0531-5565%2871%2990048-9 produce monthly?
• How much money does http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=5572739 generate?
• Learn about the earnings of http://scholar.google.com/scholar_lookup?&title=The%20influence%20of%20host%20and%20tissue%20age%20on%20life%20span%20and%20growth%20rate%20of%20serially%20transplanted%20mouse%20mammary%20gland&journal=Exp.%20Gerontol.&doi=10.1016%2F0531-5565%2871%2990048-9&volume=6&pages=49-56&publication_year=1971&author=Young%2CLJ&author=Medina%2CD&author=DeOme%2CKB&author=Daniel%2CCW
• What is the earnings of http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=5504348?
• Monthly income for http://scholar.google.com/scholar_lookup?&title=Carcinogen-induced%20mammary%20tumors%20from%20preneoplastic%20nodule%20outgrowths%20in%20BALB-c%20mice&journal=Cancer%20Res.&volume=30&pages=1055-1059&publication_year=1970&author=Medina%2CD&author=DeOme%2CKB
• What's the total monthly financial gain of https://doi.org/10.1093%2Fjnci%2F55.6.1461?
• What's the income of http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=1206764?
• Get to know what's the income of http://scholar.google.com/scholar_lookup?&title=Growth%20of%20human%20normal%20and%20neoplastic%20mammary%20tissues%20in%20the%20cleared%20fat%20pad%20of%20the%20nude%20mouse&journal=J.%20Natl.%20Cancer%20Inst.&doi=10.1093%2Fjnci%2F55.6.1461&volume=55&pages=1461-1466&publication_year=1975&author=Outzen%2CHC&author=Custer%2CRP
• Check the income stats for https://doi.org/10.1002%2Fijc.2910410513
• How much money does http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=3366492 make?
• How much cash flow does http://scholar.google.com/scholar_lookup?&title=Transplantation%20of%20human%20breast%20epithelia%20to%20mammary-gland-free%20fat-pads%20of%20athymic%20nude%20mice%3A%20influnence%20of%20mammatrophic%20hormones%20of%20breast%20epithelia&journal=Int.%20J.%20Cancer&doi=10.1002%2Fijc.2910410513&volume=41&pages=713-719&publication_year=1988&author=Sheffield%2CLG&author=Welsch%2CCW have monthly?
• What's the revenue for https://doi.org/10.1210%2Fen.2002-220570?
• How much money does http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=12446616 make?
• How much revenue does http://scholar.google.com/scholar_lookup?&title=A%20novel%20method%20for%20growing%20human%20breast%20epithelium%20in%20vivo%20using%20mouse%20and%20human%20mammary%20fibroblasts&journal=Endocrinology&doi=10.1210%2Fen.2002-220570&volume=143&pages=4886-4896&publication_year=2002&author=Parmar%2CH bring in?
• How much does https://doi.org/10.1073%2Fpnas.0401064101 bring in each month?
• How much income is http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=15051869 earning monthly?
• Get to know http://www.ncbi.nlm.nih.gov/pmc/articles/PMC387357's earnings
• How much does http://scholar.google.com/scholar_lookup?&title=Reconstruction%20of%20functionally%20normal%20and%20malignant%20human%20breast%20tissues%20in%20mice&journal=Proc.%20Natl.%20Acad.%20Sci.%20U.S.A.&doi=10.1073%2Fpnas.0401064101&volume=101&pages=4966-4971&publication_year=2004&author=Kuperwasser%2CC rake in every month?
• Learn about the earnings of https://doi.org/10.1210%2Fendo-108-1-120
• What's http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=6780309's gross income?
• What's the income generated by http://scholar.google.com/scholar_lookup?&title=The%20hormonal%20basis%20of%20reproductive%20defects%20in%20athymic%20mice%3A%20diminished%20gonadotropin%20concentration%20in%20prepubertal%20females&journal=Endocrinology&doi=10.1210%2Fendo-108-1-120&volume=108&pages=120-126&publication_year=1981&author=Rebar%2CRW&author=Morandini%2CIC&author=Petze%2CJE&author=Erickson%2CGF each month?
• https://link.springer.com/doi/10.1007/978-1-4615-4295-7_6's financial summary
• How much profit does http://scholar.google.com/scholar_lookup?&title=Methods%20in%20Mammary%20Gland%20Biology%20and%20Breast%20Cancer%20Research&doi=10.1007%2F978-1-4615-4295-7_6&pages=67-74&publication_year=2000&author=Young%2CLJT generate?
• How much income is http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=10728684 earning monthly?
• How much income does http://scholar.google.com/scholar_lookup?&title=Irradiated%20mammary%20gland%20stroma%20promotes%20the%20expression%20of%20tumorigenic%20potential%20by%20unirradiated%20epithelial%20cells&journal=Cancer%20Res.&volume=60&pages=1254-1260&publication_year=2000&author=Barcellos-Hoff%2CMH&author=Ravani%2CSA have?
• How much does https://doi.org/10.1158%2F0008-5472.CAN-03-2464 rake in every month?
• How much income is http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=15126362 earning monthly?
• Learn how profitable http://scholar.google.com/scholar_lookup?&title=Radiation%20to%20stromal%20fibroblasts%20increases%20invasiveness%20of%20pancreatic%20cancer%20cells%20through%20tumor-stromal%20interactions&journal=Cancer%20Res.&doi=10.1158%2F0008-5472.CAN-03-2464&volume=64&pages=3215-3222&publication_year=2004&author=Ohuchida%2CK is on a monthly basis
• How much profit is https://doi.org/10.1158%2F0008-5472.CAN-05-0703 making per month?
• What's the revenue for http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=16061655?
• How much does http://scholar.google.com/scholar_lookup?&title=Cellular%20mechanisms%20for%20low-dose%20ionizing%20radiation-induced%20perturbation%20of%20the%20breast%20tissue%20microenvironment&journal=Cancer%20Res.&doi=10.1158%2F0008-5472.CAN-05-0703&volume=65&pages=6734-6744&publication_year=2005&author=Tsai%2CKKC&author=Chuang%2CEY-Y&author=Little%2CJB&author=Yuan%2CZM bring in each month?
• Learn how profitable https://link.springer.com/doi/10.1007/978-1-4615-4295-7_7 is on a monthly basis
• Learn about the earnings of http://scholar.google.com/scholar_lookup?&title=Methods%20in%20Mammary%20Gland%20Biology%20and%20Breast%20Cancer%20Research&doi=10.1007%2F978-1-4615-4295-7_7&pages=75-86&publication_year=2000&author=Rasmussen%2CSB&author=Young%2CLJT&author=Smith%2CGH
• How much profit is https://citation-needed.springer.com/v2/references/10.1038/nprot.2006.31?format=refman&flavour=references making per month?
• What are the total earnings of https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=David%20A%20Proia?
• What's the financial gain of https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22David%20A%20Proia%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en?
• How much cash flow does https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Charlotte%20Kuperwasser have monthly?
• How much revenue does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Charlotte%20Kuperwasser%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en generate?
• How much revenue does https://s100.copyright.com/AppDispatchServlet?title=Reconstruction%20of%20human%20mammary%20tissues%20in%20a%20mouse%20model&author=David%20A%20Proia%20et%20al&contentID=10.1038%2Fnprot.2006.31©right=Springer%20Nature%20Limited&publication=1754-2189&publicationDate=2006-06-27&publisherName=SpringerNature&orderBeanReset=true bring in?
• Revenue of https://citation-needed.springer.com/v2/references/10.1038/nprot.2006.31?format=refman&flavour=citation
• Discover the revenue of https://doi.org/10.1186/s12964-021-00798-9
• How profitable is https://doi.org/10.1007/s00441-021-03545-1?
• How much income is https://doi.org/10.1038/s41467-021-27401-6 earning monthly?
• Explore the financials of https://doi.org/10.1038/s41374-018-0159-8
• Revenue of https://doi.org/10.1007/s10911-019-09439-x
• What's the total monthly financial gain of https://www.protocols.io/?
• How much revenue does https://www.natureindex.com/ generate?
• How much does http://www.naturechina.com make?
• Get to know what's the income of https://www.natureasia.com/ja-jp

Analytics and Tracking {📊}

• Google Tag Manager

Libraries {📚}

• Prism.js
• Zoom.js

Emails and Hosting {✉️}

Mail Servers:

• mxa-002c5801.gslb.pphosted.com
• mxb-002c5801.gslb.pphosted.com

Name Servers:

• pdns1.ultradns.net
• pdns2.ultradns.net
• pdns3.ultradns.org
• pdns4.ultradns.org
• pdns5.ultradns.info
• pdns6.ultradns.co.uk

CDN Services {📦}

• Crossref