
DOI . ORG {
}
Title[redir]:
MMTV-PyMT and Derived Met-1 Mouse Mammary Tumor Cells as Models for Studying the Role of the Androgen Receptor in Triple-Negative Breast Cancer Progression | Discover Oncology
Description:
Triple-negative breast cancer (TNBC) has a faster rate of metastasis compared to other breast cancer subtypes, and no effective targeted therapies are currently FDA-approved. Recent data indicate that the androgen receptor (AR) promotes tumor survival and may serve as a potential therapeutic target in TNBC. Studies of AR in disease progression and the systemic effects of anti-androgens have been hindered by the lack of an AR-positive (AR+) immunocompetent preclinical model. In this study, we identified the transgenic MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mouse mammary gland carcinoma model of breast cancer and Met-1 cells derived from this model as tools to study the role of AR in breast cancer progression. AR protein expression was examined in late-stage primary tumors and lung metastases from MMTV-PyMT mice as well as in Met-1 cells by immunohistochemistry (IHC). Sensitivity of Met-1 cells to the AR agonist dihydrotestosterone (DHT) and anti-androgen therapy was examined using cell viability, migration/invasion, and anchorage-independent growth assays. Late-stage primary tumors and lung metastases from MMTV-PyMT mice and Met-1 cells expressed abundant nuclear AR protein, while negative for estrogen and progesterone receptors. Met-1 sensitivity to DHT and AR antagonists demonstrated a reliance on AR for survival, and AR antagonists inhibited invasion and anchorage-independent growth. These data suggest that the MMTV-PyMT model and Met-1 cells may serve as valuable tools for mechanistic studies of the role of AR in disease progression and how anti-androgens affect the tumor microenvironment.
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Keywords {π}
cells, cancer, breast, met, cell, pubmed, article, expression, google, scholar, mmtvpymt, androgen, receptor, usa, mouse, tumors, tumor, tnbc, model, metastatic, cas, human, jrk, data, primary, mammary, fig, media, progression, enza, antagonists, tissue, invasion, central, protein, metastases, metastasis, growth, university, fbs, dht, migration, response, lung, viability, enzalutamide, dmso, role, triplenegative, potential,
Topics {βοΈ}
horseradish peroxidase-conjugated anti-rabbit health r01 ca187733-01a1 late-stage mmtv-pymt tumors triple-negative breast cancer effective targeted therapies anchorage-independent growth assays mmtv-pymt mammary tumor biotinylated goat anti-rabbit stem-cell program late-stage primary tumors biotinylated goat anti-rat anti-mouse igg antibodies mmtv-pymt mouse model akt-phosphorylation dependent upregulation end-stage metastases found providing mmtv-pymt tissue targeted therapies mda-mb-453 cell lines mmtv-pymt primary tumors androgen-dependent gene expression single-cell analysis reveals anchorage-independent growth anti-tumor immune response anti-androgen therapy multiple mammary tumors pymt-positive primary tumors anti-androgen therapies breast cancer subtypes human breast cancer primary mammary tumors gene signature similar steroid hormone receptors anti-androgens affect national cancer institute transgenic mouse models mouse monoclonal antibody mmtv-pymt lung metastases related subjects human cell lines breast cancer progression immunocompetent mouse models metastatic breast cancer metastatic breast cancer breast cancer cells tissue culture core breast cancer patients multiple molecular subtypes metastatic breast carcinoma human prostate cancer privacy choices/manage cookies
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headline:MMTV-PyMT and Derived Met-1 Mouse Mammary Tumor Cells as Models for Studying the Role of the Androgen Receptor in Triple-Negative Breast Cancer Progression
description:Triple-negative breast cancer (TNBC) has a faster rate of metastasis compared to other breast cancer subtypes, and no effective targeted therapies are currently FDA-approved. Recent data indicate that the androgen receptor (AR) promotes tumor survival and may serve as a potential therapeutic target in TNBC. Studies of AR in disease progression and the systemic effects of anti-androgens have been hindered by the lack of an AR-positive (AR+) immunocompetent preclinical model. In this study, we identified the transgenic MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mouse mammary gland carcinoma model of breast cancer and Met-1 cells derived from this model as tools to study the role of AR in breast cancer progression. AR protein expression was examined in late-stage primary tumors and lung metastases from MMTV-PyMT mice as well as in Met-1 cells by immunohistochemistry (IHC). Sensitivity of Met-1 cells to the AR agonist dihydrotestosterone (DHT) and anti-androgen therapy was examined using cell viability, migration/invasion, and anchorage-independent growth assays. Late-stage primary tumors and lung metastases from MMTV-PyMT mice and Met-1 cells expressed abundant nuclear AR protein, while negative for estrogen and progesterone receptors. Met-1 sensitivity to DHT and AR antagonists demonstrated a reliance on AR for survival, and AR antagonists inhibited invasion and anchorage-independent growth. These data suggest that the MMTV-PyMT model and Met-1 cells may serve as valuable tools for mechanistic studies of the role of AR in disease progression and how anti-androgens affect the tumor microenvironment.
datePublished:2017-02-13T00:00:00Z
dateModified:2017-02-13T00:00:00Z
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Breast Cancer
Androgen Receptor
Enzalutamide
Androgen Receptor Expression
TNBC Cell
Oncology
Cancer Research
Surgical Oncology
Molecular Medicine
Radiotherapy
Internal Medicine
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headline:MMTV-PyMT and Derived Met-1 Mouse Mammary Tumor Cells as Models for Studying the Role of the Androgen Receptor in Triple-Negative Breast Cancer Progression
description:Triple-negative breast cancer (TNBC) has a faster rate of metastasis compared to other breast cancer subtypes, and no effective targeted therapies are currently FDA-approved. Recent data indicate that the androgen receptor (AR) promotes tumor survival and may serve as a potential therapeutic target in TNBC. Studies of AR in disease progression and the systemic effects of anti-androgens have been hindered by the lack of an AR-positive (AR+) immunocompetent preclinical model. In this study, we identified the transgenic MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mouse mammary gland carcinoma model of breast cancer and Met-1 cells derived from this model as tools to study the role of AR in breast cancer progression. AR protein expression was examined in late-stage primary tumors and lung metastases from MMTV-PyMT mice as well as in Met-1 cells by immunohistochemistry (IHC). Sensitivity of Met-1 cells to the AR agonist dihydrotestosterone (DHT) and anti-androgen therapy was examined using cell viability, migration/invasion, and anchorage-independent growth assays. Late-stage primary tumors and lung metastases from MMTV-PyMT mice and Met-1 cells expressed abundant nuclear AR protein, while negative for estrogen and progesterone receptors. Met-1 sensitivity to DHT and AR antagonists demonstrated a reliance on AR for survival, and AR antagonists inhibited invasion and anchorage-independent growth. These data suggest that the MMTV-PyMT model and Met-1 cells may serve as valuable tools for mechanistic studies of the role of AR in disease progression and how anti-androgens affect the tumor microenvironment.
datePublished:2017-02-13T00:00:00Z
dateModified:2017-02-13T00:00:00Z
pageStart:69
pageEnd:77
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Breast Cancer
Androgen Receptor
Enzalutamide
Androgen Receptor Expression
TNBC Cell
Oncology
Cancer Research
Surgical Oncology
Molecular Medicine
Radiotherapy
Internal Medicine
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