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google, scholar, pubmed, cancer, article, mammary, cell, metastasis, mouse, breast, tumor, young, model, lines, cells, middle, res, mol, biol, usa, transgenic, content, analysis, metastatic, cardiff, akt, expression, molecular, access, privacy, cookies, syngeneic, carcinoma, hunter, muller, mice, gene, oncogene, polyoma, human, kinase, medicine, publish, search, related, borowsky, namba, hodgson, tepper, mcgoldrick,

Topics {✒️}

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Questions {❓}

• RS Kerbel (1998) What is the optimal rodent model for anti-tumor drug testing?

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            pulmonary metastasis
            Sept9
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            tumor transplant
            Cancer Research
            Biomedicine
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            Surgical Oncology
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      headline:Syngeneic mouse mammary carcinoma cell lines: Two closely related cell lines with divergent metastatic behavior
      description:Two cell lines, Met-1fvb2 and DB-7fvb2, with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1fvb2 line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7fvb2 line derived from the FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) with a “double base” modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1fvb2 cells show high activation of AKT, while DB-7fvb2 cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.
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         breast
         breast carcinoma
         cell line metastasis
         comparative genomic hybridization
         ERBB2
         gene expression analysis
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         mouse mammary tumor virus long terminal repeat
         oncogene
         orthotopic
         phosphatidylinositol 3 kinase
         polyoma middle T
         pulmonary metastasis
         Sept9
         Spp1 osteopontin
         Opn
         syngeneic
         transgenic
         tumor transplant
         Cancer Research
         Biomedicine
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         Oncology
         Hematology
         Surgical Oncology
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