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Title[redir]:
Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress | Plant Molecular Biology Reporter
Description:
Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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Keywords {🔍}
article, google, scholar, drought, cas, cotton, stress, pubmed, plant, lipid, plants, transgenic, transfer, protein, sci, analysis, ltp, gossypium, tolerance, httpsdoiorgs, rashid, gene, peg, central, springer, manuscript, pakistan, biol, ahmad, mol, wang, privacy, cookies, content, information, role, hassan, access, genetic, life, authors, science, data, protection, publish, research, search, molecular, biology, production,
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month download article/chapter molecular biology drought-resistant gene taltp peg-induced osmotic stress round gourd plant lipid transfer protein peg-induced drought stress agrobacterium-mediated transformation full article pdf privacy choices/manage cookies fiber-related traits lipid transfer proteins osmotic stress tolerance drought stress induced induced drought stress plant physiol 67 plant sci 239 related subjects cotton plant remodeling lipid profiles drought adaptation drought stress tolerance tomato leaf curl plant direct 7 holds exclusive rights improves drought tolerance drought tolerance characters abiotic stress responses european economic area personal data hydrophobic interactions-suggesting oxidative damage caused santos-silva cad de santana rm ferreira-neto jrc expressed sequence tags root cdna library myb transcription factors project entitled “creation thokar niaz baig article zahra transgenic cotton plants conferring drought resilience drought genes stltp1 transgenic plants performed enhances drought tolerance conditions privacy policy increasing crop yield oliveira-silva rld de oliveira wd
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headline:Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress
description:Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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headline:Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress
description:Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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Lipid transfer protein
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