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Title:
Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress | Plant Molecular Biology Reporter
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Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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headline:Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress
description:Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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Genetic Transformation
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Plant Sciences
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headline:Deciphering the Role of Lipid Transfer Protein (LTP) in Transgenic Cotton Under PEG-Induced Drought Stress
description:Drought stress is one of the major threats to cotton production, especially during the reproductive stage, when water deficit severely reduces yield and fibre quality. In this work, the contribution of the lipid transfer protein (LTP) gene in conferring drought resilience to transgenic cotton plants has been studied. In silico analysis revealed conserved regions of LTP and a role in membrane stability, with a binding energy of -5.4 kcal/mol to palmitic acid, stabilized through hydrogen and hydrophobic interactions-suggesting its role in stress resilience. Transgenic plants containing the LTP gene were produced through Agrobacterium-mediated transformation with 4.71% transformation efficiency. Transgenic plants performed better under polyethylene glycol (PEG) induced drought at 5%, 7.5%, and 10%. The height of transgenic plants was 78%, 60%, and 45%, while it was 52.4%, 40%, and 30% in control plants at 5%, 7.5%, and 10% PEG application, respectively. Root length of transgenic plants was 6.10 cm, 6.50 cm, and 5.20 cm, while it was 2.92 cm, 3.20 cm, and 2.90 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Shoot length of transgenic plants was 4.68 cm, 4.45 cm, and 4 cm, while it was 2.83 cm, 2.10 cm, and 1,80 cm in control plants at 5%, 7.5%, and 10% PEG application, respectively. Similarly, the root-to-shoot ratio of transgenic and control plants was observed under the same applications of PEG stress as mentioned above. Real-time PCR analysis confirmed the 2–fourfold increase in LTP expression in transgenic plants as compared to controls. Hence, this work underlines the importance of LTP concerning the enhancement of drought adaptation in cotton.
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Genetic Transformation
Lipid transfer protein
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Plant Sciences
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Bioinformatics
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