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Title[redir]:
Up-Regulation of Podoplanin Involves in Neuronal Apoptosis in LPS-Induced Neuroinflammation | Cellular and Molecular Neurobiology
Description:
Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, information regarding its regulation and possible function in the central nervous system is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats and detected increased expression of PDPN in the brain cortex. Immunofluorescence indicated that PDPN was located in the neurons, but not astrocytes. Moreover, there was a concomitant up-regulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these three proteins in cortical primary neurons was decreased after knocking down PDPN by siRNA. Collectively, all these results suggested that the up-regulation of PDPN might be involved in neuronal apoptosis in neuroinflammation after LPS injection.
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Keywords {š}
article, pubmed, google, scholar, cas, cell, podoplanin, neuronal, neuroinflammation, cao, central, jiangsu, nantong, upregulation, yan, brain, expression, apoptosis, shen, zhou, pdpn, neurochem, cycle, china, privacy, cookies, content, information, research, liu, cells, development, int, department, university, peoples, republic, publish, search, molecular, song, system, lipopolysaccharide, access, activation, microglial, chen, rat, lung, affiliated,
Topics {āļø}
central nervous system month download article/chapter platelet-aggregation-inducing factor rat brain cortex microglial cell cultures peripheral lymphoid tissues full article pdf oral leukoplakia cells lipopolysaccharide-induced neuroinflammation privacy choices/manage cookies cell cycle activation related subjects innate immune system brain cortex lymphatic vessel formation lps-induced neuroinflammation lps-induced inos cell differentiation gene kainate-induced apoptosis microglia-mediated neurotoxicity venero jl common apoptotic mechanism article song neuronal cell death rat mixed glial european economic area clec-2-slp-76 signaling ras homolog enriched t-dependent areas paraventricular hypothalamic nuclei ramirez mi technology innovation programme surgical comprehensive laboratory detected increased expression entry rat access conditions privacy policy ectopic lymphoid organogenesis article cellular molecular neurobiology aims lateral ventral injection malignant astrocytic tumors maohong cao article log nanjing medical university cortical primary neurons accepting optional cookies cns injury author information authors stromal cells immune system
Questions {ā}
- Hirsch EC, Hunot S (2009) Neuroinflammation in Parkinsonās disease: a target for neuroprotection?
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headline:Up-Regulation of Podoplanin Involves in Neuronal Apoptosis in LPS-Induced Neuroinflammation
description:Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, information regarding its regulation and possible function in the central nervous system is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats and detected increased expression of PDPN in the brain cortex. Immunofluorescence indicated that PDPN was located in the neurons, but not astrocytes. Moreover, there was a concomitant up-regulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these three proteins in cortical primary neurons was decreased after knocking down PDPN by siRNA. Collectively, all these results suggested that the up-regulation of PDPN might be involved in neuronal apoptosis in neuroinflammation after LPS injection.
datePublished:2014-05-13T00:00:00Z
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Lipopolysaccharide
Neuronal apoptosis
Cell cycle re-entry
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Neurosciences
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headline:Up-Regulation of Podoplanin Involves in Neuronal Apoptosis in LPS-Induced Neuroinflammation
description:Podoplanin (PDPN) is a mucin-type transmembrane sialoglycoprotein expressed in multiple tissues in adult animals, including the brain, lungs, kidney, and lymphoid organs. Studies of this molecule have demonstrated its great importance in tumor metastasis, platelet aggregation, and lymphatic vessel formation. However, information regarding its regulation and possible function in the central nervous system is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats and detected increased expression of PDPN in the brain cortex. Immunofluorescence indicated that PDPN was located in the neurons, but not astrocytes. Moreover, there was a concomitant up-regulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these three proteins in cortical primary neurons was decreased after knocking down PDPN by siRNA. Collectively, all these results suggested that the up-regulation of PDPN might be involved in neuronal apoptosis in neuroinflammation after LPS injection.
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Lipopolysaccharide
Neuronal apoptosis
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